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在Dalbulus maidis (Hemiptera:Cicadellidae) 中的双林耐药性:遗传性,交叉耐药性和稳定性
Gabriel Silva Dias1, Eduardo Perkovski Machado1, Matheus Gerage Sacilotto1
1Department of Entomology and Acarology, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, Brazil.
Pest management science
|April 25, 2025
概括
玉米叶跳 (Dalbulus maidis) 的抗甲状腺杀虫剂耐药性是以自体,不完全主导和多基因的方式遗传的. 这种耐药性是不稳定的,并表现出对其他杀虫剂的交叉耐药性,影响了昆虫耐药性管理策略.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 抗虫剂耐药性 抗虫剂耐药性 抗虫剂耐药性
- 虫害管理 虫害管理 虫害管理
背景情况:
- 在巴西,Pyrethroids对于管理Dalbulus maidis (玉米叶) 是至关重要的.
- 在甲状腺控制中报告的失败需要了解抵抗机制.
- 选择了一种抗双林的D. maidis菌株进行实验室研究.
研究的目的:
- 调查D. maidis.中双林耐药性的遗传模式.
- 确定对其他类杀虫剂的交叉耐药性.
- 评估D. maidis种群中对双林耐药性的稳定性.
主要方法:
- 在实验室条件下选择一种抗双林的D. maidis菌株.
- 进行互交交叉和逆交叉来分析遗传.
- 测试对兰巴-西哈洛,伊米达克洛普里德,乙胺,甲基,碳硫和酸盐的交叉耐药性.
- 通过子研究评估耐药性稳定性,具有不同的耐药性/易感性比率和在没有选择压力的情况下在现场收集的种群.
主要成果:
- 与敏感菌株 (Sus) 相比,在选择的菌株 (Bif-R) 中观察到对双氨酸的3,170倍耐药性比率 (RR).
- 抗性遗传是自体的,不完全的主导的和多基因的.
- 发现了与兰巴-cyhalothrin,伊米达克洛普里德和阿塞塔米普里德 (RR300至2000倍) 的显著交叉耐药性.
- 没有检测到与甲基,碳硫或酸盐的交叉耐药性.
- 在D. maidis中抗Bifenthrin的耐药性是不稳定的,在消除选择压力后,在野外种群中LC50显著下降.
结论:
- 这项研究阐明了D. maidis.中对双林耐药性的遗传基础和遗传性.
- 这些发现突出了潜在的交叉耐药性问题,这对于杀虫剂选择至关重要.
- 耐药性的不稳定性表明,昆虫耐药性管理 (IRM) 策略可以有效地保持杀虫剂的有效性.
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