相关实验视频

Updated: May 10, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

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一个大型的,通用和模块化的DARPin-apoferritin支架可以通过cryo-EM可视化小蛋白质

Xin Lu1, Ming Yan2, Yang Cai3

  • 1Spallation Neutron Source Science Center, Chinese Academy of Sciences, Dongguan 523000, People's Republic of China.

IUCrJ
|April 25, 2025
PubMed
概括

一种新的支架策略增强了单粒子冷电子显微镜 (cryo-EM) 对中型蛋白质的研究. 这种方法克服了尺寸限制,使得对具有挑战性的蛋白质标的近原子分辨率成像成为可能.

关键词:
在DARP中的apoferritin.低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架,脚手架.小蛋白质是小的蛋白质.

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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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