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由结合蛋白QueF和QueE介导的生物吸收在Lysinibacillussp.中有一个OR-15的OR-15

Qing Xu¹, Weishi Tian¹, Hongliang Liu²

¹National Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.

Applied and environmental microbiology

|April 25, 2025

概括

在Lysinibacillus sp.中发现了新的结合蛋白QueF和QueE. 在OR-15中,它是OR-15. 这些由ArsR调节的蛋白质通过结合化物[]和酸[]来增强抗性和生物吸收.

科学领域:

微生物学微生物学
环境科学环境科学
生物化学生物化学

背景情况:

微生物是转化中的关键,ars操作中的基因对抗性至关重要.
超出ars操作的细胞内素封存机制尚未完全理解.
的环境无处不在和毒性需要了解微生物解毒路径.

研究的目的:

确定参与抗性和生物吸收的新型细菌基因和蛋白质.
为了阐明这些新发现的蛋白质对的分子封存机制.
研究控制这些结合蛋白的表达的调控网络.

主要方法:

隔离和表征抗的莱西尼菌种Lysinibacillus sp. 在OR-15中,它是OR-15.
在化物暴露下对queF和queE的基因表达分析.
在对敏感细菌中,queF和queE的异质表达.
对于QueF和QueE蛋白质的净化和结合测定.
位点定向的突变发生,以确定关键的氨基酸残留物,用于结合.
电泳运动转移试验用于研究ArsR与que operon促进体的结合.

主要成果:

莱西尼细菌 (Lysinibacillus sp.) 是一种植物. OR-15显示出显著的生物吸收能力.
queF和queE的表达是由化物[As(III) ]诱导的.
在AW3110.0中增强了queF或queE的异质表达,增强了As(III) 耐药性和生物吸收.
纯化的QueF和QueE直接结合了As(III) 和酸[As(V) ].
保存的半氨酸残留物 (QueF中的Cys101,QueE中的Cys37) 对于结合至关重要.
ArsR蛋白通过与其促进子区域结合来调节que操作子的表达.

结论:

F和QueE是新型结合蛋白,参与细菌耐药性和生物吸收.
这些蛋白质的功能独立于正规的ars operon.
ArsR作为que operon的转录调节剂,将应激与QueF/QueE表达联系起来.
这项研究揭示了微生物毒解毒和封存的新分子机制.

关键词:

莱西尼巴西勒斯 (Lysinibacillus) 是一个抗的耐药性抗的耐药性酸结合剂酸结合剂亚酸盐生物吸收生物吸收转录规则转录规则转录规则

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