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在Bacillus subtilis中新型turnagainolides及其生物合成基因集群的解

Peng Li^1,2, Shuang Han³, Min Wang⁴

¹Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Health Science Center, Ningbo University, Ningbo, Zhejiang, China.

Applied and environmental microbiology

|April 25, 2025

概括

研究人员从Bacillus subtilis LP中发现了六种turnagainolides,扩大了这些周期性depsipeptides的知识. 该研究确定了它们的生物合成基因集群和途径,为自然产品药物发现提供了洞察力.

科学领域:

自然产品化学自然产品化学
微生物学微生物学
分子生物学分子生物学
药物发现药物发现药物发现

背景情况:

甲基化物是具有多种生物活性的循环脱类,以前从各种微生物中鉴定出来.
生物合成基因集群和turnagainolides的途径以前是未知的.
微生物天然产品对于药物发现至关重要,因为它们的结构多样性和治疗潜力.

研究的目的:

为了识别和描述来自Bacillus subtilis LP. 的新型turnagainolide同源.
为了阐明生物合成基因集群和turnagainolides的途径.
研究turnagainolides在药物开发中的潜力,包括它们的生物膜抑制和细胞毒性活动.

主要方法:

使用LC-MS,NMR光谱学和莫舍衍生法分离和结构阐明甲化物.
基因组测序,遗传学分析和反SMASH预测,以确定生物合成基因集群 (tur-BGC).
基因淘汰实验证实了tur-BGC在turnagainolide生物合成中的作用.

主要成果:

从Bacillus subtilis LP.中确定了四种新的turnagainolide类原体 (D-G) 和两个已知的化合物 (A-B).
通过基因淘汰确定和验证了turnagainolides的完整生物合成基因集群 (tur-BGC).
对tur-BGC的同源蛋白质仅在细菌物种中发现,这表明了特定的进化血统.

结论:

这项研究扩大了Bacillus物种产生的循环的已知化学多样性.
已确定的生物合成途径为未来的代谢工程和turnagainolides的生产优化提供了基础.
甲基化物表现出生物膜抑制和细胞毒性活动,突出显示了它们在药物开发方面的潜力.

关键词:

细菌杆菌是一种生物活性生物活性生物合成生物合成这是一种depsipeptide.其次代谢物二次代谢物.轮回的化物.

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