周血管脂肪细胞的脂肪生成是由它们在下降胸前大动脉中的解剖位置来定义的
G Andres Contreras1, C Javier Rendon1, Alyssa Shadowens1
1Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.
Cells
|April 25, 2025
概括
胸前动脉中的周周血管脂肪组织 (PVAT) 根据解剖位置显示出不同的脂肪细胞发育和功能. 侧向PVAT (LP) 显示出比前向PVAT (AP) 更多的脂肪生成和腺素分泌.
科学领域:
- 心血管生物学 心血管生物学
- 脂肪组织生物学 脂肪组织生物学
- 血管生理学 血管生理学
背景情况:
- 心血管疾病,包括高血压,改变胸前大动脉结构.
- 周围血管脂肪组织 (PVAT) 在这些变化中的作用尚不清楚.
- 下降胸前动脉中的PVAT分布在三个不同的条带中:前 (AP) 和侧 (LP).
研究的目的:
- 调查解剖位置对脂肪细胞前体发育和胸前动脉PVAT内的功能的影响.
- 为了比较LP和APP之间的脂肪生成潜力和血管活性功能,PVAT.
- 探索发育差异和PVAT分布对脂肪细胞分化的影响.
主要方法:
- 从雄性斯普拉格-道利大鼠的LP和AP区域收集了PVAT.
- 脂肪细胞的祖先在体外被分化为脂肪细胞.
- 下一代RNA测序 (RNA-seq) 和基因组丰富分析 (GSEA) 在原生细胞和差异化脂肪细胞上进行.
- 评估了对血管激素-II和Piezo1激动剂的反应.
主要成果:
- LP的原始体表现出比AP的原始体高出1.13倍的脂肪生成率.
- LP显示脂肪生成调节剂和原体 (Col4a2,Col4a4) 的表达更高.
- 当LP细胞受到angiotensin-II的挑战时,它们保持了更高的脂生成能力和脂分泌,但对Piezo1激动剂治疗敏感.
结论:
- 在LP和AP PVAT之间存在脂生成活性,细胞外基质组成和脂分泌的特定位点差异.
- 这些发现表明,不同的PVAT储存在血管健康和疾病中具有不同的功能作用.
- 解剖位置显著影响胸前动脉中的脂肪细胞原生细胞和成熟脂肪细胞的行为.
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