基质硬度调节TGF-β1诱导的血统规范在多能血管干细胞中的基质硬度调节
Yujie Yan1, Yuhang Wang1, Julia S Chu2,3
1College of Medical Informatics, Chongqing Medical University, Chongqing 400016, China.
Cells
|April 25, 2025
概括
基质刚度和TGF-β1调节了多能血管干细胞 (MVSC) 的分化. 刚性基质促进光滑肌肉细胞标记物,而软基质促进胆基体标记物,而TGF-β1放大了这些效应.
科学领域:
- 生物医学工程 生物医学工程
- 干细胞生物学 干细胞生物学
- 血管生物学 血管生物学
背景情况:
- 多能血管干细胞 (MVSCs) 存在于血管组织中,并分化为介质细胞系.
- 血管损伤后,MVSCs被激活,分化为平滑肌细胞 (SMCs),用于血管重塑.
- 细胞外矩阵特性,如度,对MVSC分化的影响尚未完全理解.
研究的目的:
- 调查基板刚度如何影响MVSC分化.
- 确定TGF-β1在调节硬度依赖的MVSC谱系承诺中的作用.
- 阐明血管病理中MVSC分化背后的机制.
主要方法:
- 在不同硬度的基板上培养MVSC (硬与软).
- 分析细胞形态,粘附和表达谱系特异性标记物 (SMC和chondrogenic).
- 评估转化增长因子β1 (TGF-β1) 对MVSC差异化的影响.
主要成果:
- 硬基板上的MVSC显示细胞扩散,粘附和SMC标记物 (MHC,MYCD,CNN1,SMA) 的表达增加.
- 在软基板上的MVSC显示出高表达的混基标记物 (AGC1, COL2A1).
- TGF-β1增强了硬基质上的SMC标记表达和软基质上的chondrogenic标记表达.
结论:
- 基质刚度是MVSC谱系承诺的关键调节者,由细胞骨重组调解.
- TGF-β1充当生物化学放大剂,调节硬度依赖的MVSC差异化.
- 这些发现提供了对MVSC在血管重塑,动脉样硬化和再生中的作用的机制性见解.
更多相关视频
11:38Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
Published on: September 14, 2021
3.3K
07:49Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
11.2K
相关概念视频
01:16TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K
01:24Regulation of Angiogenesis and Blood Supply
2.5K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.5K
01:26Stem Cell Niche
4.9K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
4.9K
01:21Lineage Commitment
2.9K
Commitment is the process whereby stem cells:
2.9K
01:19Multipotency of Hematopoietic Stem Cells
3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K