22043:注射通过激活抗氧化剂系统和调节抗压基因,促进番茄幼苗的盐耐受性
Guangyan Hu1,2, Zhongjuan Zhao1,2, Yanli Wei1,2
1Shandong Province Key Laboratory of Applied Microbiology, Ecology Institute of Shandong Academy of Sciences, Qilu University of Technology, Jinan 250103, China.
Journal of fungi (Basel, Switzerland)
|April 25, 2025
概括
菌Trichoderma asperellum 22043可以提高番茄植物的盐耐受性. 它通过增强抗氧化系统和调节抗压基因,改善了盐应激下的生长和生存.
科学领域:
- 植物科学 植物科学
- 农业微生物学 农业微生物学
- 压力生理学 压力生理学
背景情况:
- 盐应激显著阻碍作物生产率,特别是在像番茄这样的必需作物中.
- 通过传统的育种方法开发耐盐品种带来了相当大的挑战.
- 微生物接种剂提供了一个有希望的替代策略,用于增强植物对非生物压力的弹性.
研究的目的:
- 调查Trichoderma asperellum 22043在改善番茄植物的盐耐受性方面的疗效.
- 阐明T. asperellum增强抗压力的潜在生理和分子机制.
- 评估T. asperellum对受盐影响的番茄苗木关键生长参数和压力指标的影响.
主要方法:
- 番茄种子经过和没有接种T. asperellum 22043子悬浮液的盐应激 (50和100毫米NaCl) 受到盐应激.
- 评估的发芽率,幼苗的存活率,植物的高度和叶绿素含量.
- 测量了麦隆迪甲 (MDA) 和素水平,作为氧化应激和透调整的指标.
- 分析了参与反应性氧物种 (ROS) 清理,离子运输和水素素活性的基因的转录水平.
主要成果:
- 在盐应激下,T. asperellum 22043注射显著增加了种子发芽和苗木生存率.
- 与未受注射的对照组相比,注射植物的植物高度和叶绿素含量增加.
- 在T. asperellum处理的植物中,马隆迪甲和普林的积累减少,这表明氧化损伤和透应激较低.
- 阿斯佩雷勒姆菌调节了ROS清除酶基因和与Na+转运器和水素相关的基因的表达.
结论:
- 番茄树苗Trichoderma asperellum 22043有效地提高了番茄树苗的耐盐性.
- 这种真菌通过激活植物的抗氧化剂防御系统来赋予抗压力.
- T. asperellum 调节应激反应基因的表达,包括输送物和水族素的基因,以在盐水条件下维持细胞平衡.
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