转录组分析显示,细胞壁调节途径参与了Pleurotus ostreatus菌体对不同热应力的耐受性
Ludan Hou1,2, Jingyi Wang1, Tonglou Li1
1College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Jinzhong 030801, China.
Journal of fungi (Basel, Switzerland)
|April 25, 2025
概括
高温通过改变细胞结构和酶活动来抑制Pleurotus ostreatus菌株的成长. RNA测序确定了参与热应激反应的关键基因,特别是细胞壁和细胞膜功能,为改善培养提供了目标.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物科学 植物科学
- 生物化学 生物化学
背景情况:
- (Pleurotus ostreatus) 是一种主要种植的食用.
- 高温环境对其农业种植产生负面影响.
- 了解菌体热应激反应对于优化种植至关重要.
研究的目的:
- 为了研究 Pleurotus ostreatus 菌根对热应激反应的分子机制.
- 识别受高温影响的差异表达基因 (DEG) 和关键生物通路.
- 发现潜在的候选基因来增强P. ostreatus的耐热性.
主要方法:
- 在28°C的初始生长后,P. ostreatus的细胞受到热应激 (32°C和36°C).
- 进行RNA测序 (RNA-seq) 来分析基因表达变化.
- 用定量实时PCR (qRT-PCR) 来验证RNA-seq对选定基因的结果.
主要成果:
- 热应激显著抑制了菌根的生长,破坏了细胞膜,使细胞壁变厚,并降低了胆酶和β-1,3-葡萄糖酶的活动.
- 通过RNA-seq检测,在36°C时发现了2118个DEG,在32°C时发现了458个DEG,这两种情况都有328个DEG的共同点.
- 丰富分析突出了参与过氧化代谢,氧化脱氧酶活性,ATP水解和细胞壁组成的基因,并为每个温度压力确定了特定的基因组.
结论:
- 在热应激的反应中,Pleurotus ostreatus表现出显著的分子和生理变化.
- 与细胞壁结构,膜完整性和代谢过程相关的基因对于耐热性至关重要.
- 该研究确定了候选基因 (例如,疏水性蛋白质,乳糖酶),这些基因可以作为开发耐热菌株的目标.
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