一种SNARE蛋白质EvSec22调节了Esteya vermicola中状细胞的生长,应激耐受性和纳米杀菌性病原性

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Updated: May 10, 2025

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

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Published on: February 19, 2019

Jingjie Yuan¹, Run Zou¹, Xuan Peng¹

¹The College of Life Science, Chongqing Normal University, Chongqing 401331, China.

Journal of fungi (Basel, Switzerland)

|April 25, 2025

概括

埃斯蒂亚白 EvSec22基因对其感染和控制松线虫的能力至关重要. 删除该基因会损害真菌生长和耐压能力,突出显示它在生物控制中的作用.

科学领域:

菌类学菌类学是指菌类学.
植物病理学植物病理学
分子生物学分子生物学

背景情况:

由Bursaphelenchus xylophilus引起的松病 (PWD) 在全球摧毁了针叶树林.
埃斯蒂亚维米科拉 (Esteya vermicola) 是一种线虫菌,显示出对PWD进行生物控制的潜力.
由SNARE蛋白质介导的膀运输对真菌病原性至关重要.

研究的目的:

调查Esteya vermicola中EvSec22基因在Bursaphelenchus xylophilus感染期间的功能.
了解EvSec22在真菌生长,致病性和耐压力方面的作用.

主要方法:

对Esteya vermicola CBS115803进行基因组测序,以确定EvSec22基因.
基因淘汰方法来创建一个 ΔEvSec22 突变.
感染测定和突变体的表型分析.

主要成果:

删除EvSec22显著降低了Esteya vermicola对Bursaphelenchus xylophilus的致病性.
EvSec22突变体表现出抑制的细胞生长,减少的结合和异常的隔膜间距.
突变菌株对透性和氧化应激的耐受性降低.

结论:

这种EvSec22基因对于Esteya vermicola对Bursaphelenchus xylophilus的毒性至关重要.
在E. vermicola中,EvSec22在调节真菌生长和非生物应激耐受性方面发挥着作用.
这项研究提供了SNARE蛋白在真菌生物控制剂中的功能.

关键词:

这就是Bursaphelenchus xylophilus.埃斯蒂亚维米科拉 (Esteya vermicola) 是一种紫色的植物.它们是SNARE蛋白质.生物控制的真菌真菌病原性病原性病原性

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