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对循环介导同热放大试验 (LAMP) Eazyplex®的评估Pneumocystis jirovecii

Ulrike Scharmann¹, Lisa Kirchhoff¹, Jan Buer¹

¹Institute of Medical Microbiology, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.

Journal of fungi (Basel, Switzerland)

|April 25, 2025

概括

循环介导的同热放大 (LAMP) 试验可以检测重症患者的肺囊炎肺炎 (PcP). 然而,它的低敏感性意味着负面结果需要进一步测试Pneumocystis jirovecii.

科学领域:

医学诊断医学诊断医学诊断
菌类学菌类学是指菌类学.
传染性疾病传染性疾病

背景情况:

肺囊性肺炎 (PcP) 是一种严重的机会性感染.
准确和快速的PcP诊断方法至关重要,特别是在危急病患者.
循环介导同热放大 (LAMP) 提供了一个潜在的快速诊断工具.

研究的目的:

评估商业上可用的LAMP试验,用于诊断肺囊性肺炎 (PcP).
在重症患者中,评估LAMP试验与qPCR相比的诊断性能.

主要方法:

在95名患者的109个下呼吸道样本上测试了商用eazyplex® LAMP测试.
定量PCR (qPCR) 作为比较的黄金标准.
计算了灵敏度,特异性,正预测值 (PPV) 和负预测值 (NPV).

主要成果:

该LAMP测试显示了68%的灵敏度,86%的特异性,99%的PPV和16%的NPV.
所有已证实PcP的患者使用LAMP测定结果呈阳性.
在时间到阳性和真菌负载之间观察到较弱的相关性 (r2 = 0.5653).

结论:

阳性LAMP结果强烈表明,在疑似感染的患者中PcP.
由于灵敏度低,负的LAMP结果并不排除PcP,需要进一步的分子研究.
该LAMP试验适用于诊断高临床怀疑的患者的PcP,而不是仅用于查目的.

关键词:

一个灯的灯光.肺囊病毒的发病情况菌性感染菌性感染分子诊断的分子诊断.肺部感染肺部感染 qPCRR 是一个很好的方法.

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相关概念视频

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.