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使用基于结构特定碎片的定量并列质谱测量来探索β-1,3/1,6-葡萄糖的体内命运

Shuying Xu¹, Jiale Hao¹, Chunyan Ye¹

¹Key Laboratory of Marine Drugs (Ministry of Education), Shandong Key Laboratory of Glycoscience and Glycotherapeutics, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.

|April 25, 2025

概括

一种新的方法可以在生物样本中准确量化β-1,3/1,6-葡萄糖 (BG136). 这一突破支持BG136用于免疫抗瘤治疗的临床开发.

科学领域:

生物化学生物化学
药理学药理学是指药理学的学科.
分析化学分析化学

背景情况:

β-葡萄糖 (BG136) 显示出免疫抗瘤治疗的前景,但缺乏敏感的量化方法.
复杂的多糖结构和生物干扰阻碍了临床环境中的准确测量.

研究的目的:

开发一种高度敏感和可靠的分析策略,用于在生物矩阵中量化BG136.
为了能够精确测量BG136用于临床应用和药理动力学研究.

主要方法:

针对BG136.6的目标去聚合和衍生.
使用超高性能液态染色学三重四极联质谱法 (UHPLC-MS/MS) 进行寡糖糖异构体指纹检测.
基于特定的三糖标记物 (Glc-β1,6-Glc-β1,3-Glc) 的绝对量化.

主要成果:

一种经过验证,敏感的方法,用于在各种生物样本中定量BG136.
在体内成功跟踪BG136,包括细胞吸收,释放动力学和药理动力学.
提供了关于BG136体内行为的见解,从给药到排出.

结论:

开发的UHPLC-MS/MS策略为在复杂的生物矩阵中量化BG136提供了一个实用的解决方案.
这种方法支持BG136作为免疫抗瘤治疗的临床进展.
通过提供关键的药理动力学数据,使BG136第一期临床试验的成功执行成为可能.

关键词:

在 UPLC-MS/MS 中使用.在活体中,命运是命运.方法验证方法的验证方法.一个标记性寡糖的标志物.这是一种β-1, 3/1, 6-葡萄糖.

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