Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Amyloid Fibrils03:03

Amyloid Fibrils

9.0K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
9.0K
Protein and Protein Structure02:15

Protein and Protein Structure

77.3K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
77.3K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

3.7K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.7K
Protein Denaturation01:28

Protein Denaturation

3.6K
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
3.6K
Protein Folding01:22

Protein Folding

116.6K
Overview
116.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The solution supramolecular structure of α2 → 8 polysialic acid suggests a structural cause for its low immunogenicity.

FEBS letters·2025
Same author

Scanning electrochemical probe microscopy: general discussion.

Faraday discussions·2025
Same author

Spectroelectrochemistry and light active process at nanointerface: general discussion.

Faraday discussions·2025
Same author

Systems nanoelectrochemistry from single entity to ensemble: general discussion.

Faraday discussions·2025
Same author

Confined nanopore electrochemistry: general discussion.

Faraday discussions·2025
Same author

Models for an Ultraviolet-C Research and Development Consortium.

Journal of research of the National Institute of Standards and Technology·2024

相关实验视频

Updated: May 10, 2025

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.3K

溶液中的炭毒素致命因子没有蛋白质的结晶结构.

Kenneth A Rubinson1,2, John J Kasianowicz3,4

  • 1Department of Biochemistry and Molecular Biology, Wright State University, Dayton, OH 45435, USA.

Toxins
|April 25, 2025
PubMed
概括

小角度中子散射揭示了Bacillus anthracis致命因子 (LF) 采用了独特的溶液结构,与其晶体形式显著不同. 这一发现挑战了从晶体学到溶液状态的蛋白质结构的直接建模.

关键词:
没有SANS,就没有SANS.炭病是一种炭病.致命因素是致命的因素.解决方案结构 解决方案结构

更多相关视频

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
10:30

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Published on: December 27, 2013

5.3K
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

7.4K

相关实验视频

Last Updated: May 10, 2025

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.3K
A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators
10:30

A High-throughput-compatible FRET-based Platform for Identification and Characterization of Botulinum Neurotoxin Light Chain Modulators

Published on: December 27, 2013

5.3K
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

7.4K

科学领域:

  • 生物化学 生物化学
  • 结构生物学 结构生物学
  • 微生物学 微生物学

背景情况:

  • 炭杆菌分泌外毒素,包括致命因子 (LF),保护性抗原 (PA) 和瘤因子 (EF).
  • 切割到PA63的PA83促进LF和EF进入宿主细胞,导致细胞死亡.
  • 在蛋白质数据库中存在许多LF的晶体结构,主要显示一致的单体或结合形式.

研究的目的:

  • 通过小角度中子散射 (SANS) 确定Bacillus anthracis致命因子 (LF) 的溶液结构.
  • 将LF的溶液结构与其已知的晶体结构进行比较.
  • 评估从晶体数据预测溶液结构的可行性.

主要方法:

  • 采用小角度中子散射 (SANS) 来测量D2O溶液中LF的平衡结构.
  • 散射数据是用一个并列管状形状建模的,以确定分子尺寸.
  • 晶体结构数据 (PDB 1pwu) 也被建模用于比较.

主要成果:

  • LF的溶液结构最好用尺寸为12 Å × 49 Å × 129 Å的平行管模拟.
  • 这种溶液形状与从晶体结构中得出的尺寸 (30 Å × 48 Å × 104 Å) 显著不同.
  • 从溶液结构中计算的分子量与LF的已知单体重量一致.

结论:

  • 与其晶体结构相比,LF的溶液结构呈现出相当大的尺寸差异.
  • 从晶体形式直接物理建模溶液结构不足以准确地表示溶液中LF的状态.
  • 这些发现强调了基于溶液的结构研究对于理解生物背景中的蛋白质功能的重要性.