集成和可连接的基于纸张的芯片生物传感器,用于全合一的色度检测亚毒素B1
Sareh Sadat Moshirian-Farahi1, Hamidreza Rahmanian2, Jianxiong Wu3
1College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Zhejiang University, Hangzhou, 310058, China; Qingyuan County Sanheyuan Agriculture Development Co. ltd., Qingyuan, China.
Biosensors & bioelectronics
|April 25, 2025
概括
一个新的色度生物传感器通过使用纳米酶和膜封闭,以高灵敏度检测阿弗拉托辛B1 (AFB1). 这种快速的智能手机分析方法可以提高食品安全监测.
科学领域:
- 分析化学 分析化学
- 纳米技术 纳米技术
- 生物感应是一种生物感应.
背景情况:
- 非洲毒素B1 (AFB1) 对健康构成重大风险,需要敏感的检测方法.
- 现有的AFB1检测方法往往缺乏速度,灵敏度或现场适用性.
- 基于纳米酶的生物传感器为快速和敏感的分析物检测提供了有希望的替代方案.
研究的目的:
- 开发用于AFB1检测的快速,灵敏和用户友好的色度生物传感器.
- 整合一个超氧化酶模拟纳米酶与膜限制的信号放大策略.
- 使用智能手机成像实现实时,现场AFB1监控.
主要方法:
- 使用了AFB1特异的Fe3+化中孔性碳纳米圈,标有APTAMER标签,作为纳米酶.
- 采用纸质纳米纤维膜用于阿普坦体固定和反应封闭.
- 实施了基于智能手机的成像策略,用于定量信号分析.
- 杆膜封闭来放大通过纳米酶催化生成的色度信号.
主要成果:
- 实现了AFB1的广泛检测范围,从0.01到1000 ng/mL.
- 获得了AFB1.1的高灵敏度检测极限3.9 pg/mL.
- 在复杂的样本矩阵中表现出卓越的性能和稳定性,如料和药草.
- 与开放溶液方法相比,膜封闭策略显著提高了信号强度.
结论:
- 开发的色度生物传感器为AFB1检测提供了卓越的灵敏度,稳定性和操作简单性.
- 基于智能手机的平台提供了一种通用且易于使用的实时,现场食品安全监测工具.
- 这种基于纳米酶的方法在快速检测毒素以保护公共健康方面取得了重大进展.
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