在重复的妊娠损失中,homocysteine通过MAPK途径诱导子宫内膜铁
Xin Yang1, Lijie Wang2, Ruifang Wang1
1Department of Reproductive Medicine, Lanzhou University Second Hospital, Lanzhou, 730030, China.
Placenta
|April 25, 2025
概括
升高的同类半氨酸 (HCY) 可能通过通过MAPK通路诱导人类子宫内膜层细胞 (hESCs) 的铁亡,导致重复性妊娠丧失 (RPL). 这项研究揭示了将高HCY与RPL联系起来的潜在机制.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子机制的机制
- 代谢学和蛋白质学.
背景情况:
- 回复性流产 (RPL) 有复杂的原因.
- 高水平的homocysteine (HCY) 是RPL的危险因素,但其机制尚不清楚.
研究的目的:
- 阐明HCY对RPL的贡献机制.
- 研究HCY对人类子宫内膜层细胞 (hESCs) 的作用.
主要方法:
- 从具有和没有HCY的RPL患者和对照的样本中进行了代谢和蛋白质组分析.
- 在hESCs的体外测试 (细胞活力,增殖,迁移,决定化)
- 测量活性氧物种 (ROS),脂质过氧化和蛋白质表达.
主要成果:
- 在hESCs中HCY诱导的功能障碍与铁和谷甲代谢有关.
- 铁素-1 (Fer-1) 治疗逆转了HCY诱导的细胞损伤,并使ROS和脂质过氧化水平正常化.
- HCY激活了MAPK信号通路,促进了铁亡,而这被MAPK抑制逆转.
结论:
- HCY通过MAPK介导的铁,破坏hESC功能,可能导致RPL.
- 这项研究提供了高HCY和RPL之间的机制联系.
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