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MBL通过触发AKT/NF-κB/Rab5A轴来调节巨细胞酶和杀菌活性,这一轴发生在脊椎动物进化早期

Liangliang Mu¹, Jiadong Li¹, Zhanyao Lin¹

¹Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangzhou, P. R. China.

Journal of immunology (Baltimore, Md. : 1950)

|April 25, 2025

概括

尼罗河的寡合体形式曼诺结合性莱克 (MBL) 增强了细菌的结合和细胞化. 这种MBL-calreticulin复合体与CD91a一起,激活AKT/NF-κB/Rab5A信号传递,从而产生强大的抗菌免疫力.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
鱼类生物学鱼类生物学

背景情况:

曼诺结合性莱克 (MBL) 是脊椎动物的一个关键的先天性免疫分子.
在尼罗河鱼中,MBL (OnMBL) 与calreticulin (CRT) 相互作用,以促进独立于补充的细菌opsonofagocytosis.
对于OnMBL的特定寡合体结构和详细的免疫通路仍然不清楚.

研究的目的:

研究尼罗河鱼中OnMBL的寡合体结构特征.
阐明在OnMBL介导的抗菌免疫反应背后的分子机制.
确定参与OnMBL功能的受体和信号通路.

主要方法:

在细菌免疫接种后,在鱼血清中分析OnMBL寡合体形式.
评估OnMBL对细菌结合,细胞和细胞体形成的影响.
使用淘汰方法调查OnMBL与卡尔雷蒂库林 (OnCRT) 和CD91a (OnCD91a) 的相互作用.
信号通路 (AKT/NF-κB/Rab5A) 的解涉及到在OnMBL中介的细胞.

主要成果:

在Streptococcus agalactiae免疫接种后,观察到增加的三元体和四元体OnMBL水平.
较高的OnMBL寡合化增强了细菌的结合和细胞化.
与OnCD91a结合的OnMBL/CRT复合物促进了细胞分裂和细菌清除.
在MBL/CRT/CD91a的相互作用激活了AKT/NF-κB/Rab5A信号轴,增强了巨细胞的抗菌活性.

结论:

寡合体OnMBL在尼罗河鱼的先天抗菌免疫力中起着至关重要的作用.
在OnMBL/CRT/CD91a复合体激活关键信号通路,以增强细胞和细菌清除.
这项研究提供了关于早期脊椎动物中MBL介导的先天免疫的演变的见解.

关键词:

在AKT/NF-κB/Rab5A轴上这是一个MBL MBL.尼罗河鱼是尼罗河鱼.它具有杀菌活性,具有杀菌活性.发酵细胞的形成发酵细胞的形成

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