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Published on: May 7, 2013
与地球尘埃相比,月球尘埃诱导的肺毒性最小
Michaela B Smith1, Joshua Chou2, Dikaia Xenaki3
1School of Life Science, University of Technology Sydney, Ultimo, NSW 2007, Australia; Respiratory Cell and Molecular Biology Group, Woolcock Institute of Medical Research, Macquarie Park, NSW 2113, Australia.
月球尘埃模拟剂 (LMS-1,LHS-1) 毒性较低,主要是对肺细胞的物理刺激剂. 地球尘埃被证明比这些月球模拟剂更有毒和炎症.
科学领域:
- 天体生物学 天体生物学
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 人类登月任务需要了解月球尘埃的毒性.
- 以前的研究表明,月球尘埃模拟剂可以导致肺组织和细胞损伤.
- 阿波罗任务的数据表明,与月球尘埃相关的吸入风险很大.
研究的目的:
- 评估两个新的月球尘埃模拟剂LMS-1和LHS-1的毒性,来自月球海和高地地区.
- 为了比较月球尘埃模拟剂与地球空气中的颗粒物对肺细胞的影响.
- 评估月球尘埃模拟剂的炎症和细胞毒性潜力.
主要方法:
- 肺细胞系 (BEAS-2B,A549) 暴露于经过加工和未经加工的月球尘埃模拟剂 (LMS-1,LHS-1) 和地球尘埃.
- 颗粒大小分析,隔离颗粒≤2.5μm进行比较.
- 通过ELISA对炎症进行介质素-6 (IL-6) 和IL-8的测量.
- 细胞活力的评估使用试蓝色排除试验.
主要成果:
- 月球尘埃模拟剂在两种细胞类型中诱导了剂量依赖的细胞活力下降.
- 颗粒≤2.5μm导致的细胞死亡比较大颗粒 (≤1000μm).
- 地球尘埃表现出更大的毒性,并诱导了比月球尘埃模拟剂更高的细胞因子释放 (IL-6,IL-8).
结论:
- 月球尘埃模拟剂 (LMS-1,LHS-1) 主要作为物理刺激剂而不是高度毒性剂.
- 没有观察到氧化应激 (SOD2基因表达) 在月球尘埃模拟剂暴露时显著增加.
- 需要进一步的研究来确定来自月球其他地区的模拟剂的毒性.
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