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Updated: May 12, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
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模块化编码器:用于同时分析多重复合元码的R包

Martha A Sudermann1, Zachary S L Foster2, Samantha C L Dawson3

  • 1Oregon State University, Department of Botany and Plant Pathology, OSU Botany; 2701 SW CAMPUS WAY, Corvallis, Corvallis, Oregon, United States, 97331; sudermam@oregonstate.edu.

Phytopathology
|April 25, 2025
PubMed
概括

Demulticoder是一个新的R包,可以自动分析多个DNA元条码,简化复杂的社区识别. 该工具大大减少了手工步骤,确保数据质量与标准工作流程相当.

关键词:
生物信息学是一种生物信息学.计算生物学 计算生物学生态生态学 生态生态学微生物组是一个微生物组.这些是Oomycetes.技术 技术 技术 技术

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PCR - Polymerase Chain Reaction01:32

PCR - Polymerase Chain Reaction

Overview
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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¹H NMR Signal Multiplicity: Splitting Patterns

When protons A and X are coupled, their nuclear spin energy levels are slightly modified. This is because the energy required to excite proton A to a spin state parallel to proton X is slightly different from the energy required for it to become anti-parallel to spin X. Consequently, there are two possible excitation frequencies for A (A1 and A2), depending on the spin state of X, and vice versa. The mutual nature of coupling implies that the difference between frequencies A1 and A2, indicated...
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Mass Analyzers: Common Types

The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
Encoding01:19

Encoding

Information enters the brain through encoding, which is the input of information into the memory system. Once sensory information is received from the environment, the brain labels or codes it. The information is then organized with similar information and connected to existing concepts. Encoding occurs through automatic processing and effortful processing.
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