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Updated: May 10, 2025

Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens
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Retrospective MicroRNA Sequencing: Complementary DNA Library Preparation Protocol Using Formalin-fixed Paraffin-embedded RNA Specimens

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在使用snRandom-seqq的形式素固定的抛嵌入样本的单核总RNA测序.

Ziye Xu1,2, Yuexiao Lyu1, Haide Chen1

  • 1Department of Laboratory Medicine of The First Affiliated Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, China.

Nature protocols
|April 25, 2025
PubMed
概括

snRandom-seq使单核RNA测序从具有挑战性的甲固定嵌 (FFPE) 组织中成为可能. 这种新的方法增强了RNA检测,并提供了一个为期4天的协议来分析存档的临床标本.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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