相关实验视频
Updated: Jul 19, 2026

12:29
mRNA Interactome Capture from Plant Protoplasts
Published on: July 28, 2017
重序和转录组分析揭示了黄瓜RR基因家族的变异和表达模式
Ke Su1,2, Wenhong Ao2, Zhaolong Sun1,2
1School of Horticulture, Anhui Agricultural University, Hefei 230036, China.
Genes
|April 26, 2025
概括
这项研究确定了18个黄瓜反应调节器 (CsRR) 基因,揭示了突变和应激反应. CsPRR2在水果颜色和Fusarium oxysporum耐药性方面发挥了双重作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 农作物科学 农作物科学
背景情况:
- 黄瓜 (Cucumis sativus L.) 是一个全球重要的经济作物.
- 响应调节器 (RRs) 对植物生长,发育和应激反应至关重要.
研究的目的:
- 为了全面描述黄瓜反应调节器 (CsRR) 基因.
- 使用集成数据调查 CsRR 基因的变异和功能意义.
主要方法:
- 综合分析了182个重新测序和转录组数据集.
- 18个CsRR基因的识别和分类为A-ARR,B-ARR和PRR.
- 进行RT-qPCR实验以确认功能意义.
主要成果:
- 鉴定了18个CsRR基因;重新测序揭示了关键突变.
- 转录组数据显示,五个基因对非生物压力作出反应,四个基因对生物压力作出反应.
- 在水果颜色调节和对Fusarium oxysporum (Foc) 的抵抗方面,CsPRR2表现出双重功能.
结论:
- 综合再测序和转录基因数据提供了黄瓜中CsRR基因的全面表征.
- 这项研究为未来研究黄瓜中CsRR基因的功能机制奠定了基础.
关键词:
在 CsRRR 中.富沙氧化 (Fusarium oxysporum) f. sp. 这种植物有很多种. 黄瓜- (Foc) 的使用黄瓜黄瓜的使用方法转录组 (transcriptome) 是一个转录组.变化的变化变化变化.更多相关视频
10:40Comprehensive Workflow for the Genome-wide Identification and Expression Meta-analysis of the ATL E3 Ubiquitin Ligase Gene Family in Grapevine
Published on: December 22, 2017
08:33Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
相关概念视频
Cis-regulatory Sequences
9.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
9.6K
Alternative RNA Splicing
20.8K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
20.8K
RNA-seq
9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K