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分散大B细胞淋巴瘤 (DLBCL) 中的微RNA:具有预后潜力的生物标志物

Yuliya A Veryaskina^1,2, Sergei E Titov^1,3, Igor B Kovynev⁴

¹Laboratory of Molecular Genetics, Department of the Structure and Function of Chromosomes, Institute of Molecular and Cellular Biology, SB RAS, Novosibirsk 630090, Russia.

|April 26, 2025

概括

这项研究确定了特定的microRNAs (miRNAs) 作为扩散性大B细胞淋巴瘤 (DLBCL) 预后的潜在生物标志物. 上调的miRNA-23a,miRNA-125a和miRNA-100表明DLBCL患者的生存率更好.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

扩散性大B细胞淋巴瘤 (DLBCL) 呈现出影响瘤生长,治疗反应和预后的异质性.
遗传和表观遗传因素有助于DLBCL的复杂性质.
准确的预后标记对于优化DLBCL患者管理至关重要.

研究的目的:

确定可以提高扩散型大B细胞淋巴瘤 (DLBCL) 患者的预后准确度的微RNA (miRNA).
研究特定miRNAs在预测DLBCL患者结果中的作用.

主要方法:

微RNA测序是在DLBCL患者的骨髓样本上进行的.
实时RT-PCR用于分析淋巴结 (LN) 和BM样本中的miRNA表达水平.
卡普兰-梅尔生存分析被用来将miRNA表达与患者生存相关联.

主要成果:

在预后不佳的DLBCL患者中观察到几个miRNAs (miRNA-10b, -100, -125a, -125b, -126, -143, -23a, let-7a) 的表达减少.
上调的miRNA-23a,miRNA-125a和miRNA-100与更好的整体存活率相关.
与非癌症对照人群相比,DLBCL BM样本中检测到miRNA-151a,miRNA-148b,miRNA-192,PD-L1,TIMP1,TOP2A和TP53的水平升高.

结论:

miRNA-23a,miRNA-125a和miRNA-100是DLBCL的潜在预后生物标志物.
特定的miRNA和基因 (miRNA-151a,miRNA-148b,miRNA-192,PD-L1,TIMP1,TOP2A,TP53) 的改变表达表明骨髓参与DLBCL,即使没有明显的病理迹象.

关键词:

生物标志物生物标志物扩散大的B细胞淋巴瘤.这是一个微型RNA.

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