重组酵母通过精密发酵产生含有植物血红蛋白的秘密生产
Ha-Neul Bae1, Geun-Hyung Kim1, Seung-Oh Seo1,2
1Department of Food Science and Biotechnology, Seoul National University of Science and Technology, Seoul 01811, Republic of Korea.
Foods (Basel, Switzerland)
|April 26, 2025
概括
精密发酵可以可持续地生产植物血红蛋白 (LegHb),用于植物性肉类替代品. 重组酵母菌株实现了高产量,克服了传统的提取限制.
科学领域:
- 生物技术和食品科学 生物技术和食品科学
- 微生物发酵 微生物发酵
- 蛋白质工程是指蛋白质工程.
背景情况:
- 植物性蛋白质血红蛋白 (LegHb) 为植物性肉类替代品提供红色和肉味.
- 传统的LegHb提取方法产量低,成本高,阻碍了大规模应用.
- 使用重组微生物精密发酵为LegHb生产提供了一个可持续的替代方案.
研究的目的:
- 开发一种可持续的,大规模生产方法,使用重组酵母来生产植物的血红蛋白 (LegHb).
- 通过研究血红素补充和信号基因工程的影响来优化LegHb生产.
- 为了证明生产各种植物LegHbs的可行性,超越大豆,用于食品应用.
主要方法:
- 从大豆,小豆和豆中识别和克隆植物legHb基因.
- 在*Saccharomyces cerevisiae*和*Komagatella phaffii*中使用可诱导的促进剂和信号用于细胞外分泌的legHb基因的过度表达.
- 优化发酵条件,包括补充血红素和信号的修改,然后净化分泌的LegHb.
主要成果:
- 在复合酵母中达到高水平的植物血红蛋白:在*S. cerevisiae*中达到398.1 mg/L,在*K. phaffii*中达到1652.7 mg/L.
- 补充血红素显著提高了两个酵母菌株的LegHb产量.
- 信号基因工程进一步改善了植物LegHbs.的分泌物生产.
结论:
- 在复合酵母中精密发酵是一种可行的策略,用于高产量的植物黑血球蛋白.
- 血红素的可用性和信号的选择是优化LegHb产量的关键因素.
- 这项研究成功地产生了多样化的植物LegHbs,为植物性食品行业提供了天然色素和风味增强剂.
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