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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: May 10, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

在超性微阵列芯片上的皮科尺度数字PCR用于多目标检测.

Qingyue Xian^1,2, Jie Zhang², Yu Ching Wong³

¹Academy of Interdisciplinary Studies, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong.

|April 26, 2025

概括

这项研究引入了用于数字聚合酶连锁反应 (dPCR) 的新型超性微阵列芯片. 创新的设计提高了效率,并允许精确量化点,如乙型肝炎病毒 (HBV) DNA.

关键词:

POCT POCT 在线观看在 dPCR 中使用 dPCR.水友性水友性水友性水友性水友性水友性微阵列的微阵列

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Published on: November 15, 2017

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Last Updated: May 10, 2025

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ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza

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科学领域:

生物技术是生物技术.
微流体学微流体学
分子诊断学分子诊断学

背景情况:

目前的数字聚合酶连锁反应 (dPCR) 技术面临的挑战是设备大小,样品负载和反应单元的一致性.
滴滴合并和有限的分区大小阻碍了现有的dPCR平台的效率和准确性.

研究的目的:

开发一种用于数字聚合酶链反应 (dPCR) 的新型超性微阵列芯片.
克服现有的dPCR装置的局限性,通过使样品负载和反应单元形成均.
为了证明芯片对各种核酸目标的绝对定量检测的能力.

主要方法:

设计了一个超性微阵列芯片,利用微电机系统 (MEMS) 技术.
采用了水力动力学原理,在封闭通道内形成均的120-pL反应单元.
集成的快速热循环和实时成像,用于高效的dPCR分析.

主要成果:

在不同度下实现了乙型肝炎病毒 (HBV) 质体的绝对定量检测.
成功执行了癌症突变基因片段和参考基因的多重检测.
展示了一组统一的独立反应单元阵列,表明高的测试效率.

结论:

开发的超性微阵列芯片为数字聚合酶连锁反应 (dPCR) 提供了简化和高效的平台.
该技术显示出敏感和准确的分子诊断的巨大潜力.
这种平台非常适合于临床检测 (POCT) 应用,特别是在癌症诊断方面.