相关实验视频

Updated: May 10, 2025

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Published on: March 1, 2022

结合连接和关联映射方法,研究向日中垂直抗性的遗传结构

Juan F Montecchia^1,2, Mónica I Fass¹, Matías Domínguez³

¹Instituto de Agrobiotecnología y Biología Molecular-IABIMO-INTA-CONICET, Instituto de Biotecnología, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, INTA, Hurlingham B1686, Argentina.

Plants (Basel, Switzerland)

|April 26, 2025

概括

研究人员确定了向日Verticillium Wilt和叶 (SVW) 耐药性的关键遗传区域. 10号染色体上的一个主要的定量特征位置 (QTL) 显著影响了抗病能力,有助于向日繁殖的努力.

科学领域:

植物病理学植物病理学
遗传学遗传学是一个
农业科学农业科学

背景情况:

由*Verticillium dahliae*引起的太阳花和叶 (SVW) 是对太阳花生产的重大全球威胁.
开发耐药的日品种对于减轻作物损失至关重要.

研究的目的:

为了识别和验证与抗日向垂直和叶 (SVW) 相关的定量特征位点 (QTLs).
整合双亲和协会映射方法,以便对抗性局部进行可靠的识别.

主要方法:

在向日种群中利用双亲和协会映射策略.
在V. dahliae感染地区进行了9次复制的实地试验.
使用双消化限制位点相关DNA测序 (ddRADseq) 的基因型定型种群,分析了18161个SNP.

主要成果:

确定了10号染色体上的一个主要的QTL,解释了SVW疾病发病率的表型变异高达30%.
发现了17号,8号,9号,14号,13号和11号染色体上的额外QTL,与减少疾病发生率和严重程度有关.
在第10染色体上的主要QTL内定位了39个候选基因.

结论:

综合映射策略有效验证了SVW的阻力位点.
这些发现为繁殖日提供了宝贵的遗传资源,这些日具有对Verticillium Wilt和Leaf Mottle的增强抵抗力.

关键词:

这种植物名为Verticillium dahliae.双亲的 QTL 映射.农作物疾病耐药性耐药性 ddRADseqqq 的意思全基因组关联研究研究.

更多相关视频

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease

Published on: March 11, 2020

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance

Published on: August 23, 2013

相关概念视频

Dihybrid Crosses

Dihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses

Trihybrid Crosses
Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
The F1 generation plants of a trihybrid cross are heterozygous for all three traits and produce eight gametes. Upon self-fertilization, these gametes have an equal...

Light Acquisition

Light Acquisition

In order to produce glucose, plants need to capture sufficient light energy. Many modern plants have evolved leaves specialized for light acquisition. Leaves can be only millimeters in width or tens of meters wide, depending on the environment. Due to competition for sunlight, evolution has driven the evolution of increasingly larger leaves and taller plants, to avoid shading by their neighbors with contaminant elaboration of root architecture and mechanisms to transport water and nutrients.

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...