优化Bacillus amyloliquefaciens JL54的发酵条件和通过喷雾干燥制备粉末
Leilei Zhao1, Yanru Wang1, Min Pan1
1Collaborative Innovation Center of Modern Forestry in South China, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel, Switzerland)
|April 26, 2025
概括
这项研究优化了Bacillus amyloliquefaciens JL54发酵和喷雾干燥,以控制桃的衰退. 由此产生的配方实现了对Neofusicoccum laricinum病原体的54%的现场控制效率.
科学领域:
- 森林病理学和微生物生物控制.
- 农业微生物学和发酵技术.
背景情况:
- 由Neofusicoccum laricinum引起的枯病病对森林健康和生产力构成重大威胁.
- 有效的疾病管理策略对于可持续林业至关重要.
研究的目的:
- 开发一种优化的Bacillus amyloliquefaciens JL54粉末配方,用于控制桃的衰退.
- 通过优化发酵和喷雾干燥来增强细菌活力和抗菌活性.
主要方法:
- 使用统计设计来优化Bacillus amyloliquefaciens JL54.4的发酵介质和培养条件.
- 喷雾干燥参数被优化,以实现高粉末回收率.
- 进行了实地试验,以评估生物控制对桃死退的有效性.
主要成果:
- 优化的发酵使细菌数量增加了296.67倍 (4.45 × 10^9 cfu/mL).
- 实地试验表明,对桃死退的控制效率为54%.
- 优化的喷雾干燥实现了78.41%的粉末回收率.
结论:
- 乙氨基面菌 (Bacillus amyloliquefaciens JL54) 是一种强大的生物控制剂,可以防止桃的衰退.
- 喷雾干燥的B. amyloliquefaciens JL54的配方为森林疾病管理提供了可行的解决方案.
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