开发可吸入的细菌体脂质体,用于抗 Pseudomonas aeruginosa
Shruti S Sawant1, Maizbha Uddin Ahmed1, Nathan-Gautham Gantala1
1Department of Industrial and Molecular Pharmaceutics, College of Pharmacy, Purdue University, West Lafayette, IN 47907, USA.
Pharmaceutics
|April 26, 2025
概括
脂质体在雾化过程中保护菌体 (菌体),并减少细胞吸收,提高它们用于治疗Pseudomonas aeruginosa呼吸道感染的可用性.
科学领域:
- 生物技术是生物技术.
- 传染性疾病 传染性疾病
- 药物运输 药物运输 药物运输
背景情况:
- Pseudomonas aeruginosa 是呼吸道感染的主要原因,抗菌素耐药性增加需要替代治疗.
- 菌体 (菌体) 作为抗生素的替代品被探索,但它们的生存能力在制造和输送过程中可能会受到损害.
- 肺上皮细胞吸收的菌体减少了对细胞外P. aeruginosa的有效性.
研究的目的:
- 将菌体 (菌体) 封装在脂质体中,以改善对P. aeruginosa的传递和疗效.
- 评估脂质体封装对菌体活力,雾化,细胞吸收和细胞外保留的影响.
主要方法:
- 用薄膜水化方法将菌体封装在脂质体中.
- 评估了封装效率,菌体活力和受控释放.
- 在肺上皮细胞模型中评估了雾化对菌体活力和细胞吸收的影响.
主要成果:
- 通过使用低菌体标位用于水合,在最小的菌体标位损失 (0.64 log) 中实现了有利的封装 (58%).
- 脂质体配方在10小时内表现出受控释放,并在雾化过程中减少了菌体活力丧失.
- 脂质体封装减少了菌体细胞吸收的两倍,并增加了细胞外菌体保留.
结论:
- 脂质体封装保护菌体,增强它们对抗P. aeruginosa的抗菌作用的可用性.
- 脂质体显示了吸入菌体输送系统的潜力,以对抗呼吸道感染.
- 这种方法提供了一个有希望的策略,以克服药物耐药细菌的菌体治疗方面的挑战.
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