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基因组鉴定Enterococcus casseliflavus从肉牛和小牛中分离出来的基因组鉴定
Sani-E-Zehra Zaidi1, Rahat Zaheer2, Athanasios Zovoilis1
1Department of Biochemistry and Medical Genetics, University of Manitoba, 745 Bannatyne Avenue, Winnipeg, MB R3E 3P5, Canada.
Microorganisms
|April 26, 2025
概括
牛中常见的细菌Enterococcus casseliflavus对某些抗生素 (如林氏素) 呈现耐药性,但通常对其他抗生素敏感. 这项研究表明,它在牛小牛部门传播抗菌素耐药性的风险很低.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 一个健康,一个健康.
背景情况:
- 肠球菌种对人类,动物和环境健康的抗菌素耐药性 (AMR) 的健康指标至关重要.
- 在一项对肉牛和小牛的监测研究中,Enterococcus casseliflavus与其他常见的Enterococcus物种一起被发现.
研究的目的:
- 为了鉴定Enterococcus casseliflavus从肉牛和小牛中分离出来的特征.
- 了解E. casseliflavus在牛和小牛种群中的抗菌素耐药性模式中的作用.
主要方法:
- 对E. casseliflavus分离物进行了表型抗微生物敏感性测试.
- 全基因组测序被用于抗菌素耐药性基因分析.
- 进行了核心基因组遗传学分析,以评估遗传多样性和关系.
主要成果:
- 近12%的E. casseliflavus分离物表现出多种药物耐药性,主要是对林氏素.
- 所有分离物都具有固有的vanC类型的米辛抗性基因,其中vanC4XYT是最常见的.
- 对红红素 (ermB) 和四环素 (tetM) 的抗性基因不常见;对普里斯-达尔夫普里斯或西普罗夫洛克萨耐药性没有发现遗传基础.
- 遗传学分析揭示了两个分类,与宿主年龄,样本时间或农场来源没有明确的关联.
结论:
- 杆菌 (Enterococcus casseliflavus) 显示出对万科米的内在耐药性和对其他抗微生物药物的不同程度的耐药性,特别是对林科米.
- 这种物种很少携带常见的耐药基因,如 ermB 和 tetM.
- E. casseliflavus表现出显著的物种内部多样性.
- 根据这些发现,E. casseliflavus被认为是牛和小牛部门抗菌素耐药性的低风险贡献者.
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