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患病率和Mycobacterium avium亚种的基因定型在来自内蒙古,中国的羊群中发现结核病

Rong Zhang¹, Yue-Rong Lv², Bo Yang³

¹Otok Banner Animal Disease Prevention and Control Center, Ordos 016100, China.

Veterinary sciences

|April 26, 2025

概括

羊群中的甲状腺结核病 (PTB) 在内蒙古普遍存在,有Mycobacterium avium亚种. 在3.34%的样本中检测到侧结核病 (MAP). 地理位置和品种影响MAP的流行,C型和S型同时存在.

科学领域:

兽医流行病学兽医流行病学
微生物学微生物学
动物卫生动物健康

背景情况:

结核病 (PTB) 是一种慢性消耗性疾病,在反动物中是由Mycobacterium avium亚种引起的. 部分结核病 (MAP).
有效诊断,预防和控制PTB是具有挑战性的,导致畜牧业的重大经济损失.
高灵敏度和高特异性的检测方法对于管理PTB至关重要.

研究的目的:

在内蒙古境内进行首次大规模的羊群PTB流行病学调查.
进行MAP基因型鉴定,以了解不同MAP菌株的分布.
为了确定与MAP流行相关的潜在风险因素.

主要方法:

在内蒙古12个县和城市收集1585个新鲜便样本 (2022年3月 - 2024年10月).
从便样本中提取DNA.
使用双步定量聚合酶连锁反应 (qPCR) 方法检测和基因定型MAP.

主要成果:

在绵羊便样本中,MAP的总流行率为3.34% (53/1585),区域差异为0%至7.73%.
在地理区域 (东部:4.74%,中部:3.68%,西部:1.49%) 和农场类型 (小规模:3.23%,密集型:3.56%) 中,患病率有所不同.
C型MAP的患病率为2.90%,S型MAP的患病率为0.44%,两种类型都在东部和中部地区发现.

结论:

这项研究提供了第一个关于在内蒙古境内羊群中PTB的综合流行病学数据.
地理位置和绵羊品种被确定为影响MAP患病率的潜在风险因素.
在内蒙古东部和中部共存的C型和S型MAP菌株突显了PTB流行病学的复杂性.

关键词:

中国中国中国中国.这种细菌属于Mycobacterium avium亚种. 有关结核病的结核病.基因型基因型基因型患病率的流行情况.qPCRR 是一个很好的方法.

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