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Updated: May 10, 2025

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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开发多重实时光定量PCR用于水产养殖中的病毒病原体检测

Binzhe Zhang1,2,3, Yulie Qiu1,2,3, Chenxi Shi1,2,3

  • 1School of Ocean, Yantai University, Yantai 264005, China.

Veterinary sciences
|April 26, 2025
PubMed
概括

本研究介绍了一种快速,灵敏的多重实时PCR方法,用于检测水产养殖中四种关键的Vibrio病原体. 该检测准确地识别了临床样本中的单个和混合感染.

关键词:
塔克曼探测器探测器这就是Vibrio Vibrio.差异诊断是一种差异诊断.多重实时PCR复杂化

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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