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环保表面活性剂结构和类别对包裹病毒无活化的影响

Vaishali Sharma^1,2, Lynn Manchester³, Melissa Holstein⁴

¹Department of Biological Sciences, Michigan Technological University, Houghton, Michigan, USA.

Biotechnology journal

|April 26, 2025

概括

像n-nonyl-β-D-glucoside (NG) 这样的环保表面活性剂在生物处理中提供有效的病毒失活,匹配或超过Triton X-100. 盐增强了表面活性剂的有效性,使得NG成为生物制药生产的强大,可持续的替代品.

科学领域:

生物化学生物化学
生物技术是生物技术.
病毒学病毒学

背景情况:

欧盟2021年对Triton X-100的禁令需要可持续的替代品,用于生物制造中的病毒失活.
确保生物产品的安全性和质量依赖于有效的病毒灭活策略.

研究的目的:

为了比较环保葡萄糖类和氨基氧化物对Triton X-100的抗病毒功效.
为了确定生物制药生产中病毒失活的可持续表面活性剂.

主要方法:

对表面活性剂对疹病毒 (SuHV,HSV) 和逆转录病毒 (XMuLV) 的抗病毒疗效的评估.
在不同度 (1x CMC,0.5x CMC) 和盐度 (2M离子强度) 下评估表面活性剂的性能.

主要成果:

与Triton X-100相比,葡萄糖和氨基氧化物显示出同等或更高的病毒失活性.
较长的基链表面活性剂在1xCMC时对XMuLV更有效.
盐的添加增强了一些表面活性剂的抗病毒功效,但在高离子强度下降了对其他对疹病毒的抗病毒功效.

结论:

N-nonyl-β-D-glucoside (NG) 是一种强大的,环保的表面活性剂,用于病毒失活,性能优于Triton X-100.
盐通过降低临界菌度 (CMC),同时保持不活化,提高了表面活性剂的有效性.
在测试的变量中,NG的抗病毒性能一致,使其成为一个有希望的可持续替代品.

关键词:

生物制造生物制造生物制造两两的两的抗病毒药物是一种抗病毒药物.病毒清除的时间

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