使用超高性能液态染色学四极点飞行时间质谱学与多种数据处理技术相结合,快速识别丹江囊中的化学化合物
Xiaojie Fu1, Junting Zhou1, Jindong Zhao2
1Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei, China.
Journal of mass spectrometry : JMS
|April 26, 2025
概括
研究人员使用UPLC-Q-TOF/MS开发了一种新的分析方法,用于识别糖尿病传统中医药丹芝江唐囊 (DJC) 中的化学化合物. 这一策略成功表征了137种化合物,有助于进一步的研究.
科学领域:
- 分析化学 分析化学
- 药学认知是指药学上的认知.
- 传统中国医药 传统中国医药
背景情况:
- 丹芝江唐囊 (DJC) 是一种用于治疗2型糖尿病的中国传统医药.
- 关于DJC中的特定化学化合物的研究有限.
- 了解DJC的化学特征对于其治疗应用和进一步研究至关重要.
研究的目的:
- 开发和应用一种分析策略,以快速和系统地对DJC中的化学化合物进行表征.
- 通过使用先进的分析技术,全面分析DJC的化学成分.
- 为随后对DJC.的体内研究提供必要的数据.
主要方法:
- 开发一种分析策略,采用超高性能液体染色学四极飞行时间质谱法 (UPLC-Q-TOF/MS).
- 建立一个DJC特定的数据库,用于复合分析.
- 使用R语言和MZmine进行数据预处理,然后通过多种数据处理技术进行化合物识别,包括质量缺陷过 (MDF),提取离子染色体 (EIC),中性损失过器 (NLF),诊断断片离子过 (DFIF) 和直接识别.
主要成果:
- 来自DJC的137种化合物的表征,包括各种化学类,如单类,三类,黄类,类,有机酸和循环类.
- 使用参考物质对16种化合物的验证.
- 在全面的化学分析中展示开发的分析策略的有效性.
结论:
- 建立了基于UPLC-Q-TOF/MS的分析策略,可以有效和系统地描述DJC中的化学成分.
- 该研究提供了DJC的详细化学概况,为进一步调查其药理活动奠定了基础.
- 这项研究为未来的体内研究和DJC标准化提供了有价值的数据.
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