在模拟的炎症环境下,酸-氧化支架在体外调节牙纤维干细胞的反应
Caroline Anselmi1,2, Igor Paulino Mendes Soares2,3, Sarah Chang2
1Department of Morphology and Pediatric Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Brazil.
International endodontic journal
|April 26, 2025
概括
这项研究表明,聚烯酸/聚乙烯氧化物 (PCL/PEO) 支架中的奎尔 (QU) 和氧化物 (CH) 在炎症性环境中促进牙髓干细胞 (DPSC) 分化和矿化基因表达.
科学领域:
- 生物材料科学 生物材料科学
- 干细胞生物学 干细胞生物学
- 再生医学是一种再生医学.
背景情况:
- 牙再生对于牙组织的修复至关重要.
- 组织工程策略的目的是刺激介酶干细胞分化成牙芽细胞.
- 调节炎症是成功组织再生的关键.
研究的目的:
- 调查PCL/PEO支架中素 (QU) 和氧化 (CH) 对牙髓干细胞 (DPSC) 差异化的影响.
- 为了评估这些复合脚手架在一个模拟的炎症环境中的影响 in vitro.
- 评估 QU 和 CH 在牙再生应用中的潜力.
主要方法:
- 牙纸干细胞 (DPSCs) 被培养并用不同的QU度处理.
- 使用CH和QU的PCL/PEO支架是通过电制成的.
- 细胞活力,矿物化和炎症性基因表达在LPS刺激下在人工脉腔模型中被分析.
主要成果:
- 使用CH和QU的PCL/PEO支架表明了细胞相容性,并支持DPSC分化到5μM QU.
- 在炎症条件下的基因表达上调的性酸酶 (ALPL) 基因表达.
- 骨素 (OCN) 和牙亚酸蛋白 (DSPP) 基因表达在LPS刺激的,支架治疗的组中显著上调.
结论:
- 将QU和CH纳入PCL/PEO支架可以有效调节炎症反应.
- 这些复合支架增强了LPS挑战的DPSC中矿化相关基因的表达.
- 这些发现表明,在牙组织工程中,具有 QU 和 CH 载荷的 PCL/PEO 支架的潜力.
相关概念视频
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:31Osteoclasts in Bone Remodeling
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
01:29The Extracellular Matrix
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...