相关实验视频

Updated: May 10, 2025

Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management

Optimizing Extracellular Vesicle Delivery Using a Core-Sheath 3D-Bioprinted Scaffold for Chronic Wound Management

Published on: February 28, 2025

细胞和生物活性分子的双重输送用于伤口愈合应用

Petras Winkler¹, Yong Mao¹

¹Department of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA.

Molecules (Basel, Switzerland)

|April 26, 2025

概括

这项研究开发了一种使用微粒结合干细胞和抗生素进行慢性伤口愈合的双输送系统. 这种新的方法增强了细胞生长,并延长了抗菌活性,显示了改善治疗结果的希望.

科学领域:

生物材料科学生物材料科学
再生医学是一种再生医学.
伤口愈合治愈伤口愈合

背景情况:

慢性伤口由于炎症和感染而阻碍愈合,需要先进的治疗方法.
干细胞提供再生潜力,而抗生素可以对抗感染,但综合性输送具有挑战性.
凝甲基酸盐 (GelMA) 水凝正在探索干细胞输送,微粒 (MP) 显示出比散装水凝更好的细胞支持.

研究的目的:

开发一种先进的双输送系统,将人类介质干细胞 (hMSC) 和抗生素用于慢性伤口治疗.
创建能够携带hMSC和抗生素的GelMA微粒 (MP),以提高治疗效果.
研究持续的抗生素释放和干细胞与抗生素之间的协同效应.

主要方法:

凝MA被加工成微粒 (MP) 封装hMSCs,将其有效性与散装水凝 (HG) 封装进行比较.
青素/杆菌素 (PS) 抗生素被装入GelMAMP中,使用凝纳米粒子 (NP) 进行持续释放 (NP+PS(S和NP+PS(A)).
评估了细胞载体功能,对黄金色杆菌的抗菌活性和释放动力学.

主要成果:

与散装HG相比,GelMAMP支持hMSC增长和体外伤口愈合活动的增加.
装有PS的MP显示了长达8天的抗菌活性,表现优于散装HG.
存在hMSCs延长了MPs的抗菌作用,表明了协同作用.

结论:

凝MA微粒作为hMSCs的有效载体,增强细胞活力和伤口愈合潜力.
开发的双输送系统有效地整合了抗生素以保持持续的抗菌活性,延长了疗效.
这项研究证明了用于慢性伤口管理的协同干细胞-抗生素双输送系统的可行性.

关键词:

这种微粒是GelMA微粒.抗生素抗生素是一种抗生素.细胞载体细胞载体送货送货送货送货送货送货人类介质细胞干细胞这是一种水凝.纳米颗粒是一种纳米粒子.伤口愈合伤口愈合

更多相关视频

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Tissue Engineering: Construction of a Multicellular 3D Scaffold for the Delivery of Layered Cell Sheets

Published on: October 3, 2014

相关概念视频

Clinical Applications of Epidermal Stem Cells

Clinical Applications of Epidermal Stem Cells

Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...

Phases of Wound Repair

Phases of Wound Repair

Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...