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特异性蛋白1 (SP1) 在早期牛胚胎发育中发挥着重要作用

Anup K Talukder¹, Abdullah A Naib², Solomon Mamo²

¹School of Agriculture and Food Science, University College Dublin, Belfield, Dublin 4, Ireland; Department of Gynecology, Obstetrics and Reproductive Health, Gazipur Agricultural University, Gazipur, 1706, Bangladesh.

|April 26, 2025

概括

特异性蛋白1 (SP1) 对于早期牛胚胎发育至关重要,特别是在胚胎基因组激活期间. 抑制SP1阻碍了胚胎向16细胞和胚囊细胞阶段的进展,影响细胞数量.

科学领域:

生殖生物学生殖生物学
发展生物学发展生物学
分子生物学分子生物学

背景情况:

特异性蛋白1 (SP1) 转录的丰度是早期牛胚胎的特定阶段.
建议SP1在胚胎基因组激活 (EGA) 方面的作用,但尚未完全表征.

研究的目的:

在牛胚胎发育过程中 (2细胞到胚芽细胞) 描述SP1和相关基因表达.
研究SP1抑制对牛胚胎发育的影响.
为了验证SP1表达模式在体外和体内生产的胚胎.

主要方法:

在不同发育阶段对SP1和候选基因 (ACSS1,C1QBP,ATF3,MAT2A,POLD1) 的mRNA表达分析.
在体外培养牛胚胎,使用SP1抑制剂 (米特拉美A) 在不同度.
从体外和体内生长的胚胎中获得的转录组数据的分析.

主要成果:

SP1和相关基因表达在8细胞和16细胞阶段达到峰值,与EGA相吻合.
在剂量取决的方式中,SP1抑制减少了胚胎发育到16细胞和胚囊阶段.
抑制SP1导致细胞较少的囊胚细胞.

结论:

SP1在早期牛胚胎发育中起着至关重要的作用,特别是在EGA周围.
SP1活动对于向16细胞和胚胎囊细胞阶段的进展以及胚胎囊细胞增殖至关重要.

关键词:

牛的胚胎细胞囊是什么意思胚胎发育的时间胚胎基因组激活活动基因调节基因调节 SP1表达式 SP1表达式 SP1表达式

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