有益和致病细菌之间的交叉养利用真核宿主细胞衍生的酸和细菌菌形成了病原体-宿主接口环境
Darab Ghadimi1, Regina Fölster-Holst2, Sophia Blömer2
1Department of Microbiology and Biotechnology, Max Rubner-Institut, Hermann-Weigmann-Str 1, D-24103 Kiel, Germany.
Experimental and molecular pathology
|April 26, 2025
概括
肠道菌体和有益细菌通过调节宿主酸和致病细菌来减轻炎症. 这项研究揭示了它们在人类三重共同培养模型中的保护作用,突出了菌体的存在和细菌酸利用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 在炎症的肠道中增加的自由酸与致病性细菌过度生长相关.
- 肠道菌体 (菌体) 具有保护性免疫调节活性.
- 在肠道炎症中,菌体,细菌和宿主酸之间的相互作用仍然未被探索.
研究的目的:
- 研究菌体在致病性细菌,有益细菌和宿主细胞酸之间的三方相互作用中的作用.
- 建立和利用一种显著的 sialidase - 人类三重共同培养模型来模仿肠道环境.
主要方法:
- 使用人类三重共同培养模型 (T84结肠细胞,THP-1单细胞,Huh7肝细胞).
- 在存在或缺少KPP22菌体的情况下,共同培养受到Pseudomonas aeruginosa* PAO1 (病原体) 和Bifidobacterium bifidum* (有益细菌) 的挑战.
- 实验是在标准的DMEM和DMEM中进行的,并补充了2,3-dehydro-2-deoxy-N-acetylneuraminic acid (DANA),这是一个酸模拟物.
主要成果:
- *P. aeruginosa* PAO1诱导了促炎性细胞因子 (IL-6,IL-8),增加了自由酸,减少了细胞活力,并破坏了单层完整性.
- *P. aeruginosa* PAO1 的有害影响被KPP22菌体和*B. bifidum*显著减少.
- 双杆菌利用宿主衍生的酸和菌体的存在被确定为病原体-宿主界面的关键调节器.
结论:
- 肠道菌体和有益细菌,如*B. bifidum*,在缓解与致病性细菌过度生长相关的炎症方面发挥着至关重要的作用.
- 这些发现强调了菌体治疗和有益细菌的代谢活性在维持肠道平衡中的重要性.
- 这项研究为管理肠道微生物组和宿主健康的复杂相互作用提供了新的见解,在炎症的肠道环境中.
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