用加工的香通过向BSH活性细菌,改善性结肠炎,以偏好介导的GDCA水解为媒介
Wenjie Lu1, Zhenli Liu1, Zhiqian Song1
1Institute of Basic Theory for Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing, 100700, China.
Journal of ethnopharmacology
|April 26, 2025
概括
用加工的香膏通过促进有益的肠道细菌,有效治疗性结肠炎 (UC). 这个过程增强了特定胆汁酸的分解,减少炎症和改善UC症状.
科学领域:
- 药理学和微生物学 药理学和微生物学
- 胃肠病学 胃肠病学
背景情况:
- 香香传统上在中国和印度医学中用于治疗性结肠炎 (UC).
- 据信,处理的香料可以提高其在UC治疗中的疗效.
- 这种增强疗效背后的具体机制尚不清楚.
研究的目的:
- 调查加工香 (PF) 在治疗UC.治疗的机制.
- 检查胆盐化酶 (BSH) 活性细菌和甘氨酸 (GDCA) 水解的作用.
- 了解PF如何影响肠道微生物群的组成和功能.
主要方法:
- 使用硫酸盐 (DSS) 诱导的UC小鼠模型来评估PF的影响.
- 16S rRNA和元基因组测序确定了差异性的细菌和GDCA干扰.
- 超高性能液态色谱-三重四极质谱 (UHPLC-TQ-MS) 量化了GDCA水平.
- 实时定量聚合酶链反应 (RT-qPCR) 和体外实验评估了PF对BSH活性细菌的影响.
- 一个伪无菌的小鼠模型与GDCA干预验证了PF的治疗机制.
主要成果:
- 用加工的香膏 (PF) 显著减轻了UC症状 (P < 0.05或P < 0.01).
- 在Bifidobacterium longum和Lactobacillus acidophilus (P <0.01) 中,PF增强了胆盐酸酶 (BSH) 的活性.
- 这种增强促进了甘氨酸结合胆酸 (G-CBA) 的解离,特别是GDCA (P < 0.01).
- 通过调节B. longum和L. acidophilus的解离效率,PF降低了GDCA水平,减轻了GDCA诱导的UC恶化.
结论:
- 用加工的香 (PF) 显示出对性结肠炎 (UC) 的优异改善作用.
- 通过提高Bifidobacterium longum和Lactobacillus acidophilus对甘氨酸结合胆酸 (G-CBA) 的解离效率,特别是GDCA,PF可以实现这一目标.
- 这种机制突显了肠道微生物群,胆酸代谢和UC治疗之间的相互作用.
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