相关实验视频

Updated: May 10, 2025

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells HSPCs in Developing Zebrafish Embryos

Published on: November 30, 2017

不同Lyl1转录在斑马鱼原始血液形成中的作用

Jie-Hao Lin¹, Tong-Shu Yang¹, Wen-Qing Zhang¹

¹Development and Disease Medicine Basic Research Innovation Center, Ministry of Education, School of Medicine, South China University of Technology, Guangzhou 510006, China.

Yi chuan = Hereditas

|April 27, 2025

概括

这项研究揭示了LYL1基因转录的两种形式,LYL1f和LYL1s,在原始血液形成中发挥着不同的作用. LYL1s对于髓状细胞原体和颗粒细胞的产生至关重要,而LYL1f则影响巨细胞的发育.

科学领域:

发展生物学发展生物学
血液形成研究血液形成研究
基因规则基因规则

背景情况:

原始血液形成对于氧气运输和免疫系统发育至关重要.
像SCL和LYL1这样的转录因子调节了造血过程.
LYL1在原始血液形成中的特定作用尚不清楚.

研究的目的:

为了研究LYL1在原始血液形成中的功能.
识别和描述LYL1基因的不同转录.
阐明LYL1转录在斑马鱼血液形成中的不同作用.

主要方法:

对斑马鱼血细胞单细胞RNA测序 (scRNA-seq) 数据的分析.
序列对齐和补充DNA末端的5'快速放大 (5'RACE) 用于转录识别.
在斑马鱼胚胎中LYL1转录的Morpholino基敲除.
对公共scRNA-seq和RNA-seq数据集的分析.

主要成果:

LYL1基因 (lyl1s) 的一个简短的转录在斑马鱼的造血干细胞/原始细胞中得到了高度表达.
在斑马鱼,人类和小鼠中证实了LYL1的长 (lyl1f) 和短 (lyl1s) 转录.
在斑马鱼原始造血细胞中,lyl1主要转录lyl1s.
Knockdown 的 lyl1s 损害了原始髓质前体和粒细胞的生产.
对lyl1f的抑制促进了原始巨细胞的产生.

结论:

在斑马鱼和人类中,LYL1基因产生明显的长短转录 (lyl1f和lyl1s).
LYL1s在原始髓状细胞前体和粒细胞发育中起着关键作用.
LYL1f在调节原始巨细胞生产方面发挥着独特的作用.
这些发现为复杂的调节原始血液形成提供了新的见解.

关键词:

在Lyl1中使用.原始的血液形成.转录因子的转录因子斑马鱼是一种斑马鱼.

更多相关视频

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Published on: October 19, 2013

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Published on: February 22, 2019

相关概念视频

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells:

Overview of Hematopoiesis

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...

Production of Formed Elements

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...