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对人类肝细胞细胞系HHL-16的转录组分析和DNA甲基化分析,以应对阿弗拉托克素B1的反应

Hang Wu¹, Yun Yun Gong², John Huntriss³

¹School of Medicine, University of Leeds, Leeds, LS2 9JT, UK; School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, UK.

Chemico-biological interactions

|April 27, 2025

概括

阿弗拉托克素B1 (AFB1) 暴露会影响人类肝细胞中的基因表达和DNA甲基化. 这项研究揭示了AFB1.

科学领域:

肝病学肝病学是一种肝病学.
毒理学毒理学毒理学
分子生物学分子生物学

背景情况:

饮食中的亚弗拉托克素B1 (AFB1) 暴露与肝癌和免疫抑制有关.
在AFB1的健康影响背后的精确分子机制仍然不清楚.

研究的目的:

研究AFB1对人类肝细胞中的基因表达和DNA甲基化的影响.
为了确定参与AFB1毒性的分子途径.

主要方法:

使用了人类肝细胞细胞系 (HHL-16).
使用RNA测序 (RNA-Seq) 和生物信息分析.
通过二硫酸盐热测序评估DNA甲基化.

主要成果:

AFB1显著改变了576个基因的表达 (280个上调,296个下调).
丰富的途径包括细胞因子-细胞因子受体相互作用,NF-kappa B,TNF,IL-17和MAPK信号传递.
在AFB1治疗后,在CCL20CpG部位观察到显著的低甲基化.

结论:

AFB1影响炎症,生长和癌症关键的通路中的基因表达.
暴露于AFB1显然会影响肝细胞中的DNA甲基化模式.

关键词:

非洲毒素B1是什么?通过DNA甲基化.基因表达基因表达基因表达在HHL-16细胞中.路径路径路径

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