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Updated: May 12, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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通过RNase III E38A消化进行双链RNA测序,然后进行HPLC-MS/MS分析

Mark Chipley1, Brian Gau1, Cadence Allen2

  • 1Analytical Research and Development, Biotherapeutics Pharmaceutical Sciences, Pfizer Inc., Chesterfield, MO 63017, USA.

Journal of pharmaceutical sciences
|April 27, 2025
PubMed
概括

这项研究表明,一种经过修改的酶,Ribonuclease III E38A,可以帮助检测和测序双链RNA (dsRNA) 杂质在信使RNA (mRNA) 生产使用液态染色体协同质谱法 (LC-MS/MS). 这种方法有助于识别具有挑战性的dsRNA污染物.

关键词:
分析化学是一种分析化学.双链RNA (dsRNA) 是一种双链RNA.凝电泳是一种凝电泳.液体染色学 双重质谱法 (LC-MS/MS) 是一种流体染色法.传递者RNA (mRNA) 是一种传递器RNA.有氧核酸的部分.RNase III 第三部分RNase III E38A E38A 是一个字母.如果RNase如果.

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