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实验室制造的开源控制的机器人工作站,用于基于吸附剂的分散微提取低体积样本.

Guillem Peris-Pastor1, Jose Grau1, Luis Arruza2

  • 1GICAPC Research Group, Department of Analytical Chemistry, University of Valencia, Burjassot, Valencia, 46100, Spain.

Analytica chimica acta
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概括

一个新的,负担得起的,开源的机器人工作站自动化了用于实验室的基于吸附剂的分散微提取. 该系统可实现半自动化样品准备,使先进技术更容易获得.

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科学领域:

  • 分析化学 分析化学
  • 机器人技术 机器人技术 机器人技术
  • 生物化学 生物化学

背景情况:

  • 微提取技术比传统方法有优势,但往往需要繁的手工操作.
  • 对于许多实验室来说,用于样本准备的商业自动化系统的成本过高.
  • 在样本准备过程中,需要具有成本效益的自动化解决方案.

研究的目的:

  • 开发一个低成本的,开源的机器人工作站,用于半自动化基于吸附剂的分散微提取.
  • 为了解决手工样品准备和昂贵的商业自动化系统的局限性.
  • 为微提取技术提供可访问的自动化解决方案.

主要方法:

  • 使用负担得起的硬件和3D打印组件建造一个实验室制造的机器人工作站.
  • 通过开源Arduino软件控制机器人系统.
  • 该工作站用于生物样本中分析物的半自动分散微提取.

主要成果:

  • 开发的机器人工作站成功执行了基于吸附剂的半自动分散微提取.
  • 该系统被用于确定新生儿血清中的长链乙卡尼丁,使用流量注射分析-并联质谱法 (FIA-MS/MS).
  • 该方法表现出良好的灵敏度,精度和准确性,并应用于真实患者样本.

结论:

  • 本次介绍的机器人工作站是首个专门为吸剂分散微提取设计的非商业系统.
  • 该系统的开源性和低成本性质使得自动样本准备更容易获得更广泛的实验室.
  • 这一发展符合在样本准备过程中自动化趋势,与手工程序相比,提供了显著的优势.