相关实验视频
Updated: May 10, 2025

00:07
A Method to Study de novo Formation of Chromatin Domains
Published on: August 23, 2019
5.3K
EZH2的S-化改变了PRC2组合,甲基转移酶活性和EZH2的稳定性,以维持内皮平衡
Ashima Sakhuja1, Ritobrata Bhattacharyya1, Yash Tushar Katakia1
1Department of Biological Sciences, Birla Institute of Technology and Science (BITS) Pilani, Pilani Campus, Rajasthan, Pilani, India.
Nature communications
|April 27, 2025
概括
氧化 (NO) 通过内皮细胞中的S-化,改变EZH2蛋白的稳定性和功能. 这种表观遗传调节会影响细胞平衡和PRC2复合体活动.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 氧化 (NO) 是一个关键的信号分子,调节细胞功能.
- 蛋白质S-化是一种由NO介导的关键的翻译后修饰.
- EZH2蛋白在表观遗传调节和细胞过程中起着至关重要的作用.
研究的目的:
- 为了研究内皮细胞中EZH2的S-化.
- 阐明EZH2 S-化对其稳定性,功能和与PRC2复合物的相互作用的影响.
- 了解S-化依赖EZH2调节在内皮平衡中的作用.
主要方法:
- 在内皮细胞中进行S-化试验.
- 西方涂抹检测蛋白质水平和修改.
- 免疫沉用于研究蛋白质与蛋白质相互作用 (EZH2-SUZ12).
- 分子动力学模拟来分析蛋白质复合体的形成.
主要成果:
- 在内皮细胞中检测到EZH2的S-化.
- 由于SUZ12与PRC2.2.的解离,S-化导致H3K27me3水平降低.
- 诱导S-化诱导EZH2细胞质转移,无化和降解.
- 特定的氨酸残留物 (C329,C700) 被确定为S-化酶的部位,分别影响稳定性和催化活性.
- 分子动力学模拟证实,在S-化时,SUZ12与EZH2的结合受损.
结论:
- S-化是一种新的EZH2和PRC2复合物的调节机制.
- 这种调节会影响表观遗传修饰,并维持内皮细胞平衡.
- 这些发现提供了关于NO信号与内皮细胞内基因控制之间的相互作用的见解.
相关概念视频
Covalently Linked Protein Regulators
6.6K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.6K
Regulation of Nuclear Protein Sorting
2.3K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.3K
Protein Modifications in the RER
4.8K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
4.8K
Nucleosome Remodeling
8.8K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.8K
Chromatin Structure Regulates pre-mRNA Processing
6.8K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.8K
Spreading of Chromatin Modifications
8.1K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.1K

