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Updated: May 10, 2025

Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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加快的依赖于融合的内体逃生改善了递送的酶载荷的活性

Angel Luis Vázquez-Maldonado1, Teresia Chen2, Diego Rodriguez2

  • 1Department of Chemistry, University of California, Berkeley, California 94720, United States.

ACS central science
|April 28, 2025
PubMed
概括

一种新的迷你蛋白,AV5.3,通过提前实现内体逃逸,改善了治疗性蛋白质向细胞的输送. 这种增强的输送可以保持细胞质中的酶活性,成功地挽救细胞功能.

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Carrier-Mediated Transport01:06

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In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
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Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
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Vesicular transport is a cellular process that encompasses the engulfment of particles or dissolved substances by cells. It involves endocytosis, transcytosis, and exocytosis.
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The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
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