在异构血造干细胞移植后的血液病毒
Krisztina Hosszu-Fellous1, Samuel Cordey2, Stavroula Masouridi-Levrat3
1Geneva Center for Emerging Viral Diseases, Geneva University Hospitals, Geneva, Switzerland.
与HLA匹配受体相比, Haploidentical异构血型细胞移植受体 (haplo-PTCy) 通过元基因组下一代测序 (mNGS) 检测到的病毒特征较少. 然而,标准PCR测试表明,在平分PTCy患者中,感染率更高.
科学领域:
- 血液学 血液学 血液学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 哈普洛同一性全原性造血细胞移植接受者 (haplo-PTCy) 面临感染并发症,特别是病毒感染的风险增加.
- 了解这些患者的病毒组对于管理移植后结果至关重要.
研究的目的:
- 为了比较Haplo-PTCy接受者的血液病毒组与与人类白细胞抗原 (HLA) 相匹配的全原造血细胞移植接受者 (allo-HCTr) 的血液病毒组.
- 评估元基因组下一代测序 (mNGS) 在这些患者群体中识别病毒特征的实用性.
主要方法:
- 一项回顾性,倾向性得分匹配对研究包括成年哈普洛-PTCy和HLA匹配的合金-HCTr在2016年至2022年间移植.
- 在移植后的D0,D30,D90和D180收集的血液样本使用mNGS进行了分析.
- 对例行和症状驱动的聚合酶链反应 (PCR) 测试结果进行了比较.
主要成果:
- 分析了25对匹配的对. 与HLA匹配的Allo-HCTr.Tr相比,mNGS在D90和D30-D180之间的Haplo-PTCy接受者中检测到的病毒特征较少.
- 像Anelloviridae和Epstein-Barr病毒这样的特定病毒在与HLA匹配的接受者中更为普遍.
- 症状驱动的PCR测试显示,在Haplo-PTCy接受者中,常见的病毒病原体的感染率更高.
结论:
- 向PCR测试表明,在Haplo-PTCy患者中病毒感染增加,而mNGS显示,开始性病毒的流行率较低.
- 需要进一步的研究来确定常规mNGS在治疗移植受体中的临床相关性和价值.
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