大肠和神经纤维的嵌入式网络在单轴周边延伸过程中呈现辅助反应
bioRxiv : the preprint server for biology
|April 28, 2025
概括
结直肠在组织和神经纤维水平上都表现出辅助性机械反应. 这种独特的特性可能会放大机械信号,影响肠道疾病中的疼痛感知.
科学领域:
- 生物力学 生物力学
- 胃肠病学 胃肠病学
- 神经科学是一个神经科学.
背景情况:
- 了解结肠直肠机械是肠-大脑相互作用障碍中的内脏感觉的关键.
- 内膨胀会引起疼痛,但组织和神经对机械负荷的反应尚不清楚.
研究的目的:
- 在机械负荷下量化结直肠组织和感觉神经纤维的变形.
- 为了研究结直肠的多尺度机械行为.
主要方法:
- 开发了一种新的生物力学测试框架,集成单轴周长延伸和高分辨率光学成像.
- 使用数字图像相关性 (DIC) 进行大量组织变形和光成像,用于VGLUT2-标记的神经纤维延伸.
- 在定制的3D打印室中测试完整的小鼠结直肠细分.
主要成果:
- 在宏观和微观尺度上观察到一致的辅助性反应 (周长延伸期间的正轴应变).
- 结肠直肠样本在周围延伸 (平均中位数为0.1273) 期间显示出阳性轴向绿色-拉格朗奇菌株 (平均中位数为0.0177).
- 神经纤维分析显示,平均中位拉伸比为1.0631 (6.31%的延长),基于纤维方向的异质性.
结论:
- 结直肠及其神经网络表现出辅助性行为,可能放大机械和感觉信号.
- 提供了对结直肠结构功能关系的基础见解.
- 为生物灵感辅助性材料的设计提供信息.
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