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Updated: May 10, 2025

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
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快速-DASH:为多重复合的CRISPR-Cas9应用程序单日组装指导RNA阵列

Asfar Lathif Salaudeen, Nicholas Mateyko, Carl G de Boer

    bioRxiv : the preprint server for biology
    |April 28, 2025
    PubMed
    概括

    研究人员开发了一种快速的CRISPR-Cas9方法,以创建指导RNA (gRNA) 阵列,同时针对多个位置. 这种技术通过快速构建和测试gRNA库,使得高效的组合扰动研究成为可能.

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    CRISPR01:59

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    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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