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Updated: May 10, 2025

Flow Cytometric Analysis of Extracellular Vesicles from Cell-conditioned Media

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Published on: February 12, 2019

解开等离子体细胞外囊泡多样性与优化光谱流细胞计

Daniela Boselli¹, Francesca Clemente¹, Simona Di Terlizzi¹

¹Experimental Imaging Center, FRACTAL, Flow cytometry Resource, Advanced Cytometry Technical Applications Laboratory IRCCS Ospedale San Raffaele Milan Italy.

Journal of extracellular biology

|April 28, 2025

概括

光谱流细胞计提升了细胞外囊泡 (EV) 分析,揭示了新的EV亚群及其表型. 这种方法增强了对EV异质性和系统性红斑狼 (SLE) 等疾病中的潜在作用的理解.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
生物技术是生物技术.

背景情况:

细胞外囊泡 (EVs) 对于细胞间通信至关重要,但很难识别和免疫表型.
目前的方法限制了循环等离子电流器的深入分析.
需要先进的技术来破译EV异质性和功能.

研究的目的:

开发和优化下一代的光谱流细胞计工作流程,用于高维的等离子EVs的免疫类型.
识别新型EV亚群并描述它们的表面抗原表达.
应用工作流来分析系统性红斑狼 (SLE) 患者的血EV.

主要方法:

仪器设置的多步优化和21色的光谱流细胞计面板.
数据收集和无监督分析以根据表面抗原识别EV亚群.
将优化的工作流应用于SLE患者的血样本.

主要成果:

识别超出常见来源 (红细胞,血小板,白细胞,内皮细胞) 的新型EV亚群.
发现了表达激活或耗尽抗原的罕见EV子集,其中一些共表达多达五种抗原.
在SLE患者中观察到CD54+ EVs的显著增加,与循环ICAM升高相关.

结论:

谱流细胞计为破译血EV异质性提供了前所未有的分辨率.
开发的工作流允许识别以前未被识别的EV子集及其表型.
这种方法在EV研究和疾病生物标志物发现中对例行高维免疫型定型充满希望.

关键词:

一个小小的东西.抗体是一种抗体.细胞外囊泡细胞外囊泡这是一种色化物.免疫表因型定制免疫表因型血等离子体是什么?频谱流量细胞计学没有监督的分析分析.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...