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混合PET废物的高效生物工艺去聚合使用原始丁酶

Virender Kumar¹, Reinhard Wimmer¹, Cristiano Varrone¹

¹Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, 9220 Aalborg, Denmark.

|April 28, 2025

概括

这项研究引入了一种新的生物工艺,使用大肠杆菌的原始酶来分解混合塑料废物,实现PET (聚乙烯四甲) 的高脱聚合率,并展示了工业塑料回收的潜力.

科学领域:

生物技术是生物技术.
环境科学环境科学
生物化学生物化学

背景情况:

已经确定了几种能够去聚合聚乙烯二甲 (PET) 的塑料降解酶.
混合PET废物的高效脱聚合仍然是工业应用的挑战.

研究的目的:

开发和优化一种生物工艺,用于使用原始,细胞外表达酶去聚合混合PET废物.
评估不同酶 (FastPETase,LCC,LCC^ICCG) 在分解各种形式的PET废物的效率.

主要方法:

查FastPETase,LCC和LCC^ICCG酶用于无形PET粉末和薄膜的PET脱聚合.
在生物反应器中优化酶条件并培养产生LCC^ICCG的大肠杆菌.
在生物反应器中应用原始LCC^ICCG超浮剂来去聚合混合PET废物.

主要成果:

在没有pH控制的最佳条件下,LCC^ICCG在PET废物中实现了高达70%的脱聚合.
在大肠杆菌中,细胞外LCC^ICCG的产生产生了119±5毫克L^-1蛋白,其特异活性为1232±18U mg^-1.
使用原始LCC^ICCG的生物工艺在62°C的60小时内实现了混合PET废物 (MW1和MW2) 的78%和50%的产量.

结论:

通过使用原始LCC^ICCG,成功开发了一种具有成本效益和高效的混合PET废物脱聚合生物工艺.
开发的战略显示了大规模塑料废物处理和回收利用的巨大潜力.
这种方法为PET脱聚合提供了一种可访问的方法,减少了对纯化酶的依赖.

关键词:

生物过程生物过程.脱聚合脱聚合的过程混合PET废弃物混合PET废弃物

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