一个保存的Chlamydiota特异性的III型分泌系统效应因子与应激反应相关
Thomas Kozusnik1, Carole Kebbi-Beghdadi1, Silvia Ardissone1
1Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland.
克拉米迪奥塔细菌在压力下形成异常体. 在Waddlia chondrophila中,缺铁会对III型分泌系统效应器Wcw_0502进行上调,这种反应并非在所有甲状腺菌物种中存在. 热冲击也会诱导Wcw_0502的表达.
科学领域:
- 微生物学 微生物学
- 细菌应激反应的细菌应激反应
- 克拉米迪病原体的发生.
背景情况:
- 克拉米迪奥塔族的成员表现出一种保留的应激反应,涉及异常的身体形成.
- 这种应激反应背后的分子机制,特别是在暴露于各种压力因素时,仍然在很大程度上未被描述.
- 在Waddlia chondrophila中,Wcw_0502是一种III型分泌系统 (T3SS) 效应器,在铁缺乏引起的异常体形成过程中受到上调.
研究的目的:
- 为了研究wcw_0502基因及其同类在克拉米迪奥塔生物中对各种压力因素的反应中的调节.
- 阐明HrcA抑制剂和Hsp60伴侣在控制wcw_0502转录中的作用.
- 描述Wcw_0502效应蛋白的结构特征和潜在功能.
主要方法:
- 定量逆转录PCR (qRT-PCR) 用于在不同压力条件下测量wcw_0502和ctl0271RNA水平 (铁饥饿,热冲击).
- 染色体免疫沉 (ChIP) 测试用于评估HrcA和Hsp60与wcw_0502及其同类的促进子区域的结合.
- 生物信息分析以确定保存的调节元素和蛋白质域.
主要成果:
- 在*Waddlia chondrophila*异常体中,铁的饥饿诱导了wcw_0502的显著上调.
- 在铁饥饿时,这种*wcw_0502*的上调并未保留在其他甲状腺菌物种中,包括*Chlamydia trachomatis*和*Chlamydia pneumoniae*.
- 热冲击是唯一一个强烈上调*wcw_0502*及其*C. trachomatis*同类*ctl0271*的压力因素.
- 在促进区域中发现了一种保存的控制反转重复的陪伴表达 (CIRCE) 元素,表明HrcA/Hsp60介导的抑制.
- 在铁缺乏引起的异常体中观察到HrcA和Hsp60在*wcw_0502*促进体的占用率下降,与基因上调相关.
- Wcw_0502具有一种新型的囊结状域,以其对蛋白质溶解,化学和热降解的抗性而闻名.
结论:
- *wcw_0502*基因的调节是克拉米迪奥塔属内的压力和物种特异性.
- HrcA/Hsp60抑制器复合体可能通过CIRCE元件控制*wcw_0502*转录,压力诱导的解离导致上调.
- 独特的囊结状域表明Wcw_0502在保护细菌成分或在压力条件下促进宿主-病原体相互作用方面发挥作用.
更多相关视频
07:08Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
Published on: July 14, 2015
07:49Creating and Applying a Reference to Facilitate the Discussion and Classification of Proteins in a Diverse Group
Published on: August 16, 2017
相关概念视频
Conserved Binding Sites
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Multi-species Conserved Sequences
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved...
Conservation of Protein Domains
Conservation of Energy
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
Conservative Forces
