相关实验视频

Updated: May 10, 2025

A Macrophage-Tumor Spheroid Co-Invasion Assay

A Macrophage-Tumor Spheroid Co-Invasion Assay

Published on: January 24, 2025

通过单细胞分析,绘制乳腺癌瘤微环境中的免疫细胞动态和巨细胞可塑性的地图

Wang Chen¹, Siyu Zeng², Junyong Zhong³

¹Department of Pharmacy, The Affiliated Guangzhou Red Cross Hospital of Jinan University, Guangzhou, 510220, People's Republic of China.

Discover oncology

|April 28, 2025

概括

这项研究使用单细胞分析揭示了乳腺癌 (BRCA) 中的主要免疫细胞相互作用. 巨细胞亚型与癌细胞通信,影响瘤的进展,并可能指导新的治疗策略.

科学领域:

在瘤学瘤学.
免疫学免疫学免疫学
基因组学就是基因组学.

背景情况:

乳腺癌 (BRCA) 的进展受到瘤微环境的显著影响.
免疫细胞和癌细胞的相互作用对于瘤的发展和治疗反应至关重要.

研究的目的:

在BRCA中全面分析免疫细胞亚群和巨细胞亚型.
在单细胞水平上阐明BRCA瘤微环境中的功能性作用和相互作用.

主要方法:

单细胞RNA测序 (scRNA-seq) 用于详细分析.
使用了包括sc-type,infercnv,cellchat,cytotrace和monocle在内的计算工具.
分析包括免疫细胞注释,CNV分析,巨细胞亚型,相互作用网络阐明和轨迹分析.

主要成果:

在BRCA中确定了不同的免疫细胞亚群和五个巨细胞集群.
SPP1-CD44和LGALS9-CD44信号通路被确定为巨细胞和BRCA细胞之间的关键相互作用网络.
高风险组表现出增加的免疫透和更高的瘤突变负担 (TMB).

结论:

这项研究为BRCA瘤微环境中的免疫细胞和巨细胞相互作用提供了关键的见解.
这些发现提高了对BRCA瘤进展的理解,并为治疗干预提供了潜在的点.

关键词:

乳腺癌乳腺癌乳腺癌有效的细胞分裂.巨细胞是一个巨细胞.预测模型是一个预测模型.单细胞RNA测序的一个细胞.瘤微环境是一个微环境.

更多相关视频

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Real-time Imaging of Myeloid Cells Dynamics in ApcMin/+ Intestinal Tumors by Spinning Disk Confocal Microscopy

Published on: October 6, 2014

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...