Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

5.6K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
5.6K
Subcellular Fractionation01:32

Subcellular Fractionation

6.9K
The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Silk-Inspired Design and Manufacturing of Robust Plantymers.

Nature communications·2026
Same author

Entropic depletion of macromolecular solutes induces symmetry-breaking surface wrinkling in myelin figures.

Soft matter·2026
Same author

Protocol for Engineered Compositional Asymmetry Within Nanodiscs.

Membranes·2026
Same author

Microfluidic Approaches to Pickering Emulsions and Foams: Strategies, Challenges, and Promising Applications.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Driving a Stimuli-Responsive Wedge in the Packing of Phospholipid Membranes Using Bolaamphiphile Intercalants.

ACS nano·2025
Same author

Nanoscale Curvature-Facilitated Membrane Intercalation of Conjugated Oligoelectrolytes Revealed by Nanobar-Supported Lipid Bilayers.

ACS nano·2025

相关实验视频

Updated: Jun 16, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.1K

通过分离阶段分离来调节合成细胞内的生物紧缩物.

Chang Chen1, Caroline M Love2, Christopher F Carnahan3

  • 1Laboratory of Physical Chemistry and Soft Matter, Wageningen University & Research, 6708 WE Wageningen, The Netherlands.

ACS nano
|April 28, 2025
PubMed
概括

分离相位分离作为一种通用策略,通过控制分子分布和定位来调节细胞凝聚物. 阶段分离系统之间的这种相互作用为设计合成细胞和人造器官提供了新的原则.

关键词:
协类动物 协类脂质体是一种脂质体.没有膜的有机体.微流体学 在微流体学方面分离的分离相位分离.合成细胞的合成细胞.

更多相关视频

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

8.9K

相关实验视频

Last Updated: Jun 16, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.1K
Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

4.7K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

8.9K

科学领域:

  • 生物物理学的生物物理.
  • 细胞生物学 细胞生物学
  • 合成生物学 合成生物学

背景情况:

  • 细胞利用宏分子相互作用来驱动相位分离,形成动态的细胞内区.
  • 同时发生的多相分离事件对细胞组织的影响在很大程度上仍未被探索.

研究的目的:

  • 调查细胞模拟监禁中的分离和关联相分离之间的相互作用.
  • 了解这些现象如何影响细胞内分离和脂质边界相互作用.

主要方法:

  • 利用芯片上的微流体系统来封装关联和分离组件.
  • 在细胞大小的囊泡内引发外部相位分离,以模仿细胞环境.

主要成果:

  • 分离阶段形成了微域,影响了协会组件作为招募者,膜准剂和凝结启动器.
  • 多相架构创造了孤立的微环境,限制了分子通信和扩散.
  • 由于脂质膜上的等级域形成,观察到受限的全球形状转变.

结论:

  • 分离相位分离被提出为调节凝结物形成和定位的通用策略.
  • 阶段分离系统之间的相互作用为合成细胞和人造无膜有机体提供了设计原则.