相关实验视频
Updated: May 9, 2025

11:51
Potato Virus X-Based microRNA Silencing VbMS In Potato.
Published on: May 11, 2020
3.1K
细胞外囊泡介导的植物miRNA贩运调节了昆虫载体病毒感染
Qian Wang1, Hong Lu2, Xiaoyue Fan3
1State Key Laboratory of Animal Biodiversity Conservation and Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Cell reports
|April 28, 2025
概括
米细胞外囊泡 (EVs) 将微RNA (miRNAs) 传递给昆虫载体,影响树冠病毒感染. 这些植物衍生的miRNAs差异调节病毒复制和昆虫的平衡,影响传播.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 细胞外囊泡 (EVs) 通过小RNA贩运来调解细胞间和物种间的通信.
- 植物树病毒在昆虫载体中建立稳态,这对生存和传播至关重要.
- 植物EV介导的跨王国RNA干扰在昆虫病毒感染中的作用在很大程度上是未知的.
研究的目的:
- 研究植物细胞外囊泡 (EVs) 和它们封装的微RNA (miRNAs) 在媒介昆虫载体的跨王国RNA干扰中的作用.
- 阐明EV中植物miRNAs影响arbovirus复制和昆虫平衡的机制.
主要方法:
- 米细胞外囊泡 (EVs) 的分离和表征.
- 使用小RNA测序识别米EV中封装的微RNA (miRNA).
- 输送大米EVs和特定的miRNAs到中肠上皮细胞的小棕色植物 (SBPH).
- 在EV治疗后对SBPH的基因表达和病毒复制的分析.
主要成果:
- 两种米特异性的miRNAs,Osa-miR159a.1-1和Osa-miR167a,在孤立的米EV中被确定.
- 这些miRNAs成功地被运送到小棕色植物 (SBPH) 的中肠上皮细胞中,SBPH是条病毒 (RSV) 的载体.
- Osa-miR159a.1-1通过增强脂酶C的mRNA稳定性,抑制亡,促进了RSV复制.
- Osa-miR167a通过直接与病毒RNA依赖RNA聚合酶 (RdRp) 结合来抑制RSV复制.
结论:
- 植物EV介导的特定miRNAs的传递在调节昆虫载体中的树冠病毒感染方面发挥着重要作用.
- 通过Osa-miR159a.1-1和Osa-miR167a的差异调节有助于昆虫载体内的树冠病毒稳态.
- 这种跨王国RNA干扰机制对于有效的树冠病毒传播至关重要.
相关概念视频
siRNA - Small Interfering RNAs
16.3K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.3K
RNA Interference
25.7K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.7K
MicroRNAs
2.9K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
2.9K
Experimental RNAi
6.0K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.0K

